Noninvasive in Vivo Monitoring of Extracellular Vesicles

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2013 Oct 30

PMID 24166382

Citations 28

Authors

Charles P Lai

Bakhos A Tannous

Xandra O Breakefield

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) including exosomes and microvesicles are nanometer-sized vesicles released by cells to deliver lipids, cellular proteins, mRNAs, and noncoding RNAs, thereby facilitating intercellular communication without direct cell-to-cell contacts. Due to their nanoscale size, EVs have been visualized under microscopy in vitro. We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo.

Citing Articles

Research progress in tracing technology for extracellular vesicles.

Shi Y, Wang X, Zhang S, Yin H, Fan H, Tang Y Extracell Vesicles Circ Nucl Acids. 2024; 4(4):684-697.

PMID: 39697802 PMC: 11648465. DOI: 10.20517/evcna.2023.49.

Therapeutic potential of stem cells in subarachnoid hemorrhage.

Kanamaru H, Suzuki H Neural Regen Res. 2024; 20(4):936-945.

PMID: 38989928 PMC: 11438332. DOI: 10.4103/NRR.NRR-D-24-00124.

MicroRNA Nano-Shuttles: Engineering Extracellular Vesicles as a Cutting-Edge Biotechnology Platform for Clinical Use in Therapeutics.

Menjivar N, Oropallo J, Gebremedhn S, Souza L, Gad A, Puttlitz C Biol Proced Online. 2024; 26(1):14.

PMID: 38773366 PMC: 11106895. DOI: 10.1186/s12575-024-00241-6.

Radiolabelled Extracellular Vesicles as Imaging Modalities for Precise Targeted Drug Delivery.

Ashique S, Anand K Pharmaceutics. 2023; 15(5).

PMID: 37242668 PMC: 10220982. DOI: 10.3390/pharmaceutics15051426.

Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism.

Eguchi T, Lu Y, Taha E, Okusha Y Methods Mol Biol. 2022; 2582:103-126.

PMID: 36370347 DOI: 10.1007/978-1-0716-2744-0_9.

References

Al-Nedawi K, Meehan B, Kerbel R, Allison A, Rak J . Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR. Proc Natl Acad Sci U S A. 2009; 106(10):3794-9. PMC: 2656159. DOI: 10.1073/pnas.0804543106. View

Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, Wood M . Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol. 2011; 29(4):341-5. DOI: 10.1038/nbt.1807. View

Antonyak M, Li B, Boroughs L, Johnson J, Druso J, Bryant K . Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells. Proc Natl Acad Sci U S A. 2011; 108(12):4852-7. PMC: 3064359. DOI: 10.1073/pnas.1017667108. View

Tannous B, Grimm J, Perry K, Chen J, Weissleder R, Breakefield X . Metabolic biotinylation of cell surface receptors for in vivo imaging. Nat Methods. 2006; 3(5):391-6. DOI: 10.1038/nmeth875. View

Kogure T, Lin W, Yan I, Braconi C, Patel T . Intercellular nanovesicle-mediated microRNA transfer: a mechanism of environmental modulation of hepatocellular cancer cell growth. Hepatology. 2011; 54(4):1237-48. PMC: 3310362. DOI: 10.1002/hep.24504. View

Marzesco A, Janich P, Wilsch-Brauninger M, Dubreuil V, Langenfeld K, Corbeil D . Release of extracellular membrane particles carrying the stem cell marker prominin-1 (CD133) from neural progenitors and other epithelial cells. J Cell Sci. 2005; 118(Pt 13):2849-58. DOI: 10.1242/jcs.02439. View

Lai C, Breakefield X . Role of exosomes/microvesicles in the nervous system and use in emerging therapies. Front Physiol. 2012; 3:228. PMC: 3384085. DOI: 10.3389/fphys.2012.00228. View

Muralidharan-Chari V, Clancy J, Sedgwick A, DSouza-Schorey C . Microvesicles: mediators of extracellular communication during cancer progression. J Cell Sci. 2010; 123(Pt 10):1603-11. PMC: 2864708. DOI: 10.1242/jcs.064386. View

Balaj L, Lessard R, Dai L, Cho Y, Pomeroy S, Breakefield X . Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences. Nat Commun. 2011; 2:180. PMC: 3040683. DOI: 10.1038/ncomms1180. View

10.

Taylor D, Gercel-Taylor C . Exosomes/microvesicles: mediators of cancer-associated immunosuppressive microenvironments. Semin Immunopathol. 2011; 33(5):441-54. DOI: 10.1007/s00281-010-0234-8. View

11.

Al-Nedawi K, Meehan B, Micallef J, Lhotak V, May L, Guha A . Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells. Nat Cell Biol. 2008; 10(5):619-24. DOI: 10.1038/ncb1725. View

12.

Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G . Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat Med. 2012; 18(6):883-91. PMC: 3645291. DOI: 10.1038/nm.2753. View

13.

Mizrak A, Bolukbasi M, Ozdener G, Brenner G, Madlener S, Erkan E . Genetically engineered microvesicles carrying suicide mRNA/protein inhibit schwannoma tumor growth. Mol Ther. 2012; 21(1):101-8. PMC: 3538300. DOI: 10.1038/mt.2012.161. View

14.

Ohno S, Takanashi M, Sudo K, Ueda S, Ishikawa A, Matsuyama N . Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells. Mol Ther. 2012; 21(1):185-91. PMC: 3538304. DOI: 10.1038/mt.2012.180. View

15.

Mittelbrunn M, Gutierrez-Vazquez C, Villarroya-Beltri C, Gonzalez S, Sanchez-Cabo F, Gonzalez M . Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. Nat Commun. 2011; 2:282. PMC: 3104548. DOI: 10.1038/ncomms1285. View

16.

Montecalvo A, Larregina A, Shufesky W, Stolz D, Sullivan M, Karlsson J . Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes. Blood. 2011; 119(3):756-66. PMC: 3265200. DOI: 10.1182/blood-2011-02-338004. View

17.

Skog J, Wurdinger T, van Rijn S, Meijer D, Gainche L, Sena-Esteves M . Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol. 2008; 10(12):1470-6. PMC: 3423894. DOI: 10.1038/ncb1800. View

18.

Luga V, Zhang L, Viloria-Petit A, Ogunjimi A, Inanlou M, Chiu E . Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration. Cell. 2012; 151(7):1542-56. DOI: 10.1016/j.cell.2012.11.024. View

19.

Suetsugu A, Honma K, Saji S, Moriwaki H, Ochiya T, Hoffman R . Imaging exosome transfer from breast cancer cells to stroma at metastatic sites in orthotopic nude-mouse models. Adv Drug Deliv Rev. 2012; 65(3):383-90. DOI: 10.1016/j.addr.2012.08.007. View

20.

Tannous B, Kim D, Fernandez J, Weissleder R, Breakefield X . Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther. 2005; 11(3):435-43. DOI: 10.1016/j.ymthe.2004.10.016. View