SNPs of MYPN and TTN Genes Are Associated to Meat and Carcass Traits in Italian Large White and Italian Duroc Pigs

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2013 Oct 26

PMID 24158610

Citations 5

Authors

Silvia Braglia

Roberta Davoli

Andrea Zappavigna

Paolo Zambonelli

Luca Buttazzoni

Maurizio Gallo

Vincenzo Russo

Affiliations

Soon will be listed here.

Abstract

Myopalladin (MYPN) and titin (TTN) play key roles in skeletal muscle structural organisation and for this reason they may be functional candidates for meat production traits in pigs. We analysed by PCR-RFLP two polymorphisms previously identified and described in the 3'UTR of MYPN and TTN genes in a group of Italian Large White (ILW) and Italian Duroc (ID) pigs with extreme divergent estimated breeding values for some production traits. Significant allele frequency differences were observed for lean cuts (LC) at the MYPN locus in ILW breed and for visible intermuscular fat (VIF) at MYPN and TTN loci in ID breed. Moreover, association analysis between the two genes and some production traits (average daily gain, LC, backfat thickness, ham weight, feed conversion ratio and VIF) was performed in a sample of 409 ILW and 381 ID pigs. On the whole, the results showed significant effects of both genes on some of the considered traits in ID breed, and for TTN gene in ILW animals. These results indicate that both genes can be considered as candidates for meat and carcass traits in ID pigs.

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References

Wimmers K, Murani E, Te Pas M, Chang K, Davoli R, Merks J . Associations of functional candidate genes derived from gene-expression profiles of prenatal porcine muscle tissue with meat quality and muscle deposition. Anim Genet. 2007; 38(5):474-84. DOI: 10.1111/j.1365-2052.2007.01639.x. View

Tskhovrebova L, Trinick J . Titin: properties and family relationships. Nat Rev Mol Cell Biol. 2003; 4(9):679-89. DOI: 10.1038/nrm1198. View

Malek M, Dekkers J, Lee H, Baas T, Prusa K, Huff-Lonergan E . A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm Genome. 2001; 12(8):637-45. DOI: 10.1007/s003350020019. View

Bang M, Centner T, Fornoff F, Geach A, Gotthardt M, McNabb M . The complete gene sequence of titin, expression of an unusual approximately 700-kDa titin isoform, and its interaction with obscurin identify a novel Z-line to I-band linking system. Circ Res. 2001; 89(11):1065-72. DOI: 10.1161/hh2301.100981. View

Ma K, Wang K . Interaction of nebulin SH3 domain with titin PEVK and myopalladin: implications for the signaling and assembly role of titin and nebulin. FEBS Lett. 2002; 532(3):273-8. DOI: 10.1016/s0014-5793(02)03655-4. View

Castro-Ferreira R, Fontes-Carvalho R, Falcao-Pires I, Leite-Moreira A . The role of titin in the modulation of cardiac function and its pathophysiological implications. Arq Bras Cardiol. 2011; 96(4):332-9. DOI: 10.1590/s0066-782x2011005000023. View

Misztal I . FAQ for genomic selection. J Anim Breed Genet. 2011; 128(4):245-6. DOI: 10.1111/j.1439-0388.2011.00944.x. View

Duboscq-Bidot L, Xu P, Charron P, Neyroud N, Dilanian G, Millaire A . Mutations in the Z-band protein myopalladin gene and idiopathic dilated cardiomyopathy. Cardiovasc Res. 2007; 77(1):118-25. DOI: 10.1093/cvr/cvm015. View

Sato K, Yoshimura A, Kaneko T, Ukai T, Ozaki Y, Nakamura H . A single nucleotide polymorphism in 3'-untranslated region contributes to the regulation of Toll-like receptor 4 translation. J Biol Chem. 2012; 287(30):25163-72. PMC: 3408206. DOI: 10.1074/jbc.M111.338426. View

10.

Miller M, Bang M, Witt C, Labeit D, Trombitas C, Watanabe K . The muscle ankyrin repeat proteins: CARP, ankrd2/Arpp and DARP as a family of titin filament-based stress response molecules. J Mol Biol. 2003; 333(5):951-64. DOI: 10.1016/j.jmb.2003.09.012. View

11.

Barendse W, Harrison B, Bunch R, Thomas M . Variation at the Calpain 3 gene is associated with meat tenderness in zebu and composite breeds of cattle. BMC Genet. 2008; 9:41. PMC: 2474649. DOI: 10.1186/1471-2156-9-41. View

12.

Tskhovrebova L, Trinick J . Giant proteins: sensing tension with titin kinase. Curr Biol. 2008; 18(24):R1141-2. DOI: 10.1016/j.cub.2008.10.035. View

13.

Rohrer G, Keele J . Identification of quantitative trait loci affecting carcass composition in swine: II. Muscling and wholesale product yield traits. J Anim Sci. 1998; 76(9):2255-62. DOI: 10.2527/1998.7692255x. View

14.

Liu G, Jennen D, Tholen E, Juengst H, Kleinwachter T, Holker M . A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. Anim Genet. 2007; 38(3):241-52. DOI: 10.1111/j.1365-2052.2007.01592.x. View

15.

Li H, Lund M, Christensen O, Gregersen V, Henckel P, Bendixen C . Quantitative trait loci analysis of swine meat quality traits. J Anim Sci. 2010; 88(9):2904-12. DOI: 10.2527/jas.2009-2590. View

16.

Bertani G, Larsen N, Marklund S, Hu Z, Rothschild M . Rapid communication: mapping of the titin (TTN) gene to pig chromosome 15. J Anim Sci. 1999; 77(10):2857-8. DOI: 10.2527/1999.77102857x. View

17.

Guo W, Bharmal S, Esbona K, Greaser M . Titin diversity--alternative splicing gone wild. J Biomed Biotechnol. 2010; 2010:753675. PMC: 2843904. DOI: 10.1155/2010/753675. View

18.

Bang M, Mudry R, McElhinny A, Trombitas K, Geach A, Yamasaki R . Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies. J Cell Biol. 2001; 153(2):413-27. PMC: 2169455. DOI: 10.1083/jcb.153.2.413. View

19.

Kruger M, Linke W . The giant protein titin: a regulatory node that integrates myocyte signaling pathways. J Biol Chem. 2011; 286(12):9905-12. PMC: 3060543. DOI: 10.1074/jbc.R110.173260. View

20.

Szklarczyk D, Franceschini A, Kuhn M, Simonovic M, Roth A, Minguez P . The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res. 2010; 39(Database issue):D561-8. PMC: 3013807. DOI: 10.1093/nar/gkq973. View