Fundus Camera Guided Photoacoustic Ophthalmoscopy

Overview

Journal Curr Eye Res

Publisher Informa Healthcare

Specialty Ophthalmology

Date 2013 Oct 18

PMID 24131226

Citations 17

Authors

Tan Liu

Hao Li

Wei Song

Shuliang Jiao

Hao F Zhang

Affiliations

Soon will be listed here.

Abstract

Purpose: To demonstrate the feasibility of fundus camera guided photoacoustic ophthalmoscopy (PAOM) system and its multimodal imaging capabilities.

Methods: We integrated PAOM and a fundus camera consisting of a white-light illuminator and a high-sensitivity, high-speed CCD. The fundus camera captures both retinal anatomy and PAOM illumination at the same time to provide a real-time feedback when we position the PAOM illuminating light. We applied the integrated system to image rat eyes in vivo and used full-spectrum, visible (VIS), and near infrared (NIR) illuminations in fundus photography.

Results: Both albino and pigmented rat eyes were imaged in vivo. During alignment, different trajectories of PAOM laser scanning were successfully visualized by the fundus camera, which reduced the PAOM alignment time from several minutes to 30 s. In albino eyes, in addition to retinal vessels, main choroidal vessels were observed using VIS-illumination, which is similar to PAOM images. In pigmented eyes, the radial striations of retinal nerve fiber layer were visualized by fundus photography using full-spectrum illumination; meanwhile, PAOM imaged both retinal vessels and the retinal pigmented epithelium melanin distribution.

Conclusions: The results demonstrated that PAOM can be well-integrated with fundus camera without affecting its functionality. The fundus camera guidance is faster and easier comparing with our previous work. The integrated system also set the stage for the next-step verification between oximetry methods based on PAOM and fundus photography.

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References

Zhang X, Zhang H, Puliafito C, Jiao S . Simultaneous in vivo imaging of melanin and lipofuscin in the retina with photoacoustic ophthalmoscopy and autofluorescence imaging. J Biomed Opt. 2011; 16(8):080504. PMC: 3162618. DOI: 10.1117/1.3606569. View

Ramella-Roman J, Mathews S, Kandimalla H, Nabili A, Duncan D, DAnna S . Measurement of oxygen saturation in the retina with a spectroscopic sensitive multi aperture camera. Opt Express. 2008; 16(9):6170-82. DOI: 10.1364/oe.16.006170. View

Hardarson S, Stefansson E . Retinal oxygen saturation is altered in diabetic retinopathy. Br J Ophthalmol. 2011; 96(4):560-3. DOI: 10.1136/bjophthalmol-2011-300640. View

Jiao S, Jiang M, Hu J, Fawzi A, Zhou Q, Shung K . Photoacoustic ophthalmoscopy for in vivo retinal imaging. Opt Express. 2010; 18(4):3967-72. PMC: 2864517. DOI: 10.1364/OE.18.003967. View

Delori F, Webb R, Sliney D . Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices. J Opt Soc Am A Opt Image Sci Vis. 2007; 24(5):1250-65. DOI: 10.1364/josaa.24.001250. View

Wangsa-Wirawan N, Linsenmeier R . Retinal oxygen: fundamental and clinical aspects. Arch Ophthalmol. 2003; 121(4):547-57. DOI: 10.1001/archopht.121.4.547. View

Wei Q, Liu T, Jiao S, Zhang H . Image chorioretinal vasculature in albino rats using photoacoustic ophthalmoscopy. J Mod Opt. 2014; 58(21):1997-2001. PMC: 3987921. DOI: 10.1080/09500340.2011.601331. View

Kawaguchi I, Higashide T, Ohkubo S, Takeda H, Sugiyama K . In vivo imaging and quantitative evaluation of the rat retinal nerve fiber layer using scanning laser ophthalmoscopy. Invest Ophthalmol Vis Sci. 2006; 47(7):2911-6. DOI: 10.1167/iovs.05-1169. View

Goatman K . A reference standard for the measurement of macular oedema. Br J Ophthalmol. 2006; 90(9):1197-202. PMC: 1857383. DOI: 10.1136/bjo.2006.095885. View

10.

Zhang H, Puliafito C, Jiao S . Photoacoustic ophthalmoscopy for in vivo retinal imaging: current status and prospects. Ophthalmic Surg Lasers Imaging. 2011; 42 Suppl:S106-15. PMC: 3291958. DOI: 10.3928/15428877-20110627-10. View

11.

DeHoog E, Schwiegerling J . Optimal parameters for retinal illumination and imaging in fundus cameras. Appl Opt. 2008; 47(36):6769-77. DOI: 10.1364/ao.47.006769. View

12.

Liu T, Wei Q, Song W, Burke J, Jiao S, Zhang H . Near-infrared light photoacoustic ophthalmoscopy. Biomed Opt Express. 2012; 3(4):792-9. PMC: 3345807. DOI: 10.1364/BOE.3.000792. View

13.

Link D, Strohmaier C, Seifert B, Riemer T, Reitsamer H, Haueisen J . Novel non-contact retina camera for the rat and its application to dynamic retinal vessel analysis. Biomed Opt Express. 2011; 2(11):3094-108. PMC: 3207378. DOI: 10.1364/BOE.2.003094. View

14.

Liu W, Jiao S, Zhang H . Accuracy of retinal oximetry: a Monte Carlo investigation. J Biomed Opt. 2013; 18(6):066003. PMC: 3669519. DOI: 10.1117/1.JBO.18.6.066003. View

15.

Elsner A, Burns S, Weiter J, Delori F . Infrared imaging of sub-retinal structures in the human ocular fundus. Vision Res. 1996; 36(1):191-205. DOI: 10.1016/0042-6989(95)00100-e. View

16.

Hammer M, Vilser W, Riemer T, Schweitzer D . Retinal vessel oximetry-calibration, compensation for vessel diameter and fundus pigmentation, and reproducibility. J Biomed Opt. 2008; 13(5):054015. DOI: 10.1117/1.2976032. View

17.

Hammer M, Leistritz S, Leistritz L, Schweitzer D . Light paths in retinal vessel oxymetry. IEEE Trans Biomed Eng. 2001; 48(5):592-8. DOI: 10.1109/10.918598. View

18.

Song W, Wei Q, Liu T, Kuai D, Burke J, Jiao S . Integrating photoacoustic ophthalmoscopy with scanning laser ophthalmoscopy, optical coherence tomography, and fluorescein angiography for a multimodal retinal imaging platform. J Biomed Opt. 2012; 17(6):061206. PMC: 3380928. DOI: 10.1117/1.JBO.17.6.061206. View

19.

Harris A, Dinn R, Kagemann L, Rechtman E . A review of methods for human retinal oximetry. Ophthalmic Surg Lasers Imaging. 2003; 34(2):152-64. View

20.

Delori F, Goger D, Dorey C . Age-related accumulation and spatial distribution of lipofuscin in RPE of normal subjects. Invest Ophthalmol Vis Sci. 2001; 42(8):1855-66. View