Near-infrared Light Photoacoustic Ophthalmoscopy

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2012 May 11

PMID 22574266

Citations 15

Authors

Tan Liu

Qing Wei

Wei Song

Janice M Burke

Shuliang Jiao

Hao F Zhang

Affiliations

Soon will be listed here.

Abstract

We achieved photoacoustic ophthalmoscopy (PAOM) imaging of the retina with near-infrared (NIR) light illumination. A PAOM imaging system with dual-wavelength illumination at 1064 nm and 532 nm was built. We compared in vivo imaging results of both albino and pigmented rat eyes at the two wavelengths. The results show that the bulk optical absorption of the retinal pigment epithelium (RPE) is only slightly higher than that of the retinal vessels at 532 nm while it becomes more than an order of magnitude higher than that of the retinal vessels at 1064 nm. These studies suggest that although visible light illumination is suitable for imaging both the retinal vessels and the RPE, NIR light illumination, being more comfortable to the eye, is better suited for RPE melanin related investigations and diagnoses.

Citing Articles

Contrast-enhanced near-infrared photoacoustic microscopy and optical coherence tomography imaging of rat fundus.

Du F, Niu C, Zeng S, Chen J, Liu C, Dai C Nanophotonics. 2024; 13(19):3631-3646.

PMID: 39635031 PMC: 11465996. DOI: 10.1515/nanoph-2023-0872.

Intraocular reflectance of the ocular fundus and its impact on increased retinal hazard.

Fehler N, Lingenfelder C, Kupferschmid S, Hessling M Z Med Phys. 2022; 32(4):453-465.

PMID: 35618555 PMC: 9948856. DOI: 10.1016/j.zemedi.2022.03.001.

Recent Progress on Near-Infrared Photoacoustic Imaging: Imaging Modality and Organic Semiconducting Agents.

Jung D, Park S, Lee C, Kim H Polymers (Basel). 2019; 11(10).

PMID: 31623160 PMC: 6836006. DOI: 10.3390/polym11101693.

Photoacoustic Imaging with Capacitive Micromachined Ultrasound Transducers: Principles and Developments.

Chan J, Zheng Z, Bell K, Le M, Reza P, Yeow J Sensors (Basel). 2019; 19(16).

PMID: 31434241 PMC: 6720758. DOI: 10.3390/s19163617.

Retinal and choroidal imaging using integrated photoacoustic microscopy and optical coherence tomography.

Tian C, Zhang W, Nguyen V, Huang Z, Wang X, Paulus Y Proc SPIE Int Soc Opt Eng. 2019; 10474.

PMID: 31296972 PMC: 6621601. DOI: 10.1117/12.2290667.

References

Zhang H, Maslov K, Stoica G, Wang L . Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging. Nat Biotechnol. 2006; 24(7):848-51. DOI: 10.1038/nbt1220. View

Zhang X, Zhang H, Puliafito C, Jiao S . Simultaneous in vivo imaging of melanin and lipofuscin in the retina with photoacoustic ophthalmoscopy and autofluorescence imaging. J Biomed Opt. 2011; 16(8):080504. PMC: 3162618. DOI: 10.1117/1.3606569. View

Wang R, An L . Multifunctional imaging of human retina and choroid with 1050-nm spectral domain optical coherence tomography at 92-kHz line scan rate. J Biomed Opt. 2011; 16(5):050503. PMC: 3113334. DOI: 10.1117/1.3582159. View

Wei Q, Liu T, Jiao S, Zhang H . Image chorioretinal vasculature in albino rats using photoacoustic ophthalmoscopy. J Mod Opt. 2014; 58(21):1997-2001. PMC: 3987921. DOI: 10.1080/09500340.2011.601331. View

You J, Chen T, Mujat M, Park B, de Boer J . Pulsed illumination spectral-domain optical coherence tomography for human retinal imaging. Opt Express. 2009; 14(15):6739-48. DOI: 10.1364/oe.14.006739. View

Kodach V, Kalkman J, Faber D, van Leeuwen T . Quantitative comparison of the OCT imaging depth at 1300 nm and 1600 nm. Biomed Opt Express. 2011; 1(1):176-185. PMC: 3005155. DOI: 10.1364/BOE.1.000176. View

Liu T, Wei Q, Wang J, Jiao S, Zhang H . Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen. Biomed Opt Express. 2011; 2(5):1359-65. PMC: 3087592. DOI: 10.1364/BOE.2.001359. View

Song W, Wei Q, Liu T, Kuai D, Burke J, Jiao S . Integrating photoacoustic ophthalmoscopy with scanning laser ophthalmoscopy, optical coherence tomography, and fluorescein angiography for a multimodal retinal imaging platform. J Biomed Opt. 2012; 17(6):061206. PMC: 3380928. DOI: 10.1117/1.JBO.17.6.061206. View

Hughes A, Wassle H . An estimate of image quality in the rat eye. Invest Ophthalmol Vis Sci. 1979; 18(8):878-81. View

10.

Hunter J, Masella B, Dubra A, Sharma R, Yin L, Merigan W . Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy. Biomed Opt Express. 2011; 2(1):139-48. PMC: 3028489. DOI: 10.1364/BOE.2.000139. View

11.

Kellner U, Kellner S, Weinitz S . Fundus autofluorescence (488 NM) and near-infrared autofluorescence (787 NM) visualize different retinal pigment epithelium alterations in patients with age-related macular degeneration. Retina. 2010; 30(1):6-15. DOI: 10.1097/iae.0b013e3181b8348b. View

12.

Schmitz-Valckenberg S, Steinberg J, Fleckenstein M, Visvalingam S, Brinkmann C, Holz F . Combined confocal scanning laser ophthalmoscopy and spectral-domain optical coherence tomography imaging of reticular drusen associated with age-related macular degeneration. Ophthalmology. 2010; 117(6):1169-76. DOI: 10.1016/j.ophtha.2009.10.044. View

13.

N Keilhauer C, Delori F . Near-infrared autofluorescence imaging of the fundus: visualization of ocular melanin. Invest Ophthalmol Vis Sci. 2006; 47(8):3556-64. DOI: 10.1167/iovs.06-0122. View

14.

Zhou Q, Lau S, Wu D, Shung K . Piezoelectric films for high frequency ultrasonic transducers in biomedical applications. Prog Mater Sci. 2011; 56(2):139-174. PMC: 3123890. DOI: 10.1016/j.pmatsci.2010.09.001. View

15.

Thibos L, Bradley A, Still D, Zhang X, Howarth P . Theory and measurement of ocular chromatic aberration. Vision Res. 1990; 30(1):33-49. DOI: 10.1016/0042-6989(90)90126-6. View

16.

Moon S, Kim D . Ultra-high-speed optical coherence tomography with a stretched pulse supercontinuum source. Opt Express. 2009; 14(24):11575-84. DOI: 10.1364/oe.14.011575. View

17.

Hu S, Rao B, Maslov K, Wang L . Label-free photoacoustic ophthalmic angiography. Opt Lett. 2010; 35(1):1-3. PMC: 2912610. DOI: 10.1364/OL.35.000001. View

18.

Stanga P, Lim J, Hamilton P . Indocyanine green angiography in chorioretinal diseases: indications and interpretation: an evidence-based update. Ophthalmology. 2003; 110(1):15-21; quiz 22-3. DOI: 10.1016/s0161-6420(02)01563-4. View

19.

Jiao S, Jiang M, Hu J, Fawzi A, Zhou Q, Shung K . Photoacoustic ophthalmoscopy for in vivo retinal imaging. Opt Express. 2010; 18(4):3967-72. PMC: 2864517. DOI: 10.1364/OE.18.003967. View

20.

Zhang X, Zhang H, Jiao S . Optical coherence photoacoustic microscopy: accomplishing optical coherence tomography and photoacoustic microscopy with a single light source. J Biomed Opt. 2012; 17(3):030502. PMC: 3380948. DOI: 10.1117/1.JBO.17.3.030502. View