Biallelic Genome Modification in F(0) Xenopus Tropicalis Embryos Using the CRISPR/Cas System

Overview

Journal Genesis

Specialties Biology
Molecular Biology

Date 2013 Oct 15

PMID 24123579

Citations 107

Authors

Ira L Blitz

Jacob Biesinger

Xiaohui Xie

Ken W Y Cho

Affiliations

Soon will be listed here.

Abstract

Gene inactivation is an important tool for correlation of phenotypic and genomic data, allowing researchers to infer normal gene function based on the phenotype when the gene is impaired. New and better approaches are needed to overcome the shortfalls of existing methods for any significant acceleration of scientific progress. We have adapted the CRISPR/Cas system for use in Xenopus tropicalis and report on the efficient creation of mutations in the gene encoding the enzyme tyrosinase, which is responsible for oculocutaneous albinism. Biallelic mutation of this gene was detected in the F0 generation, suggesting targeting efficiencies similar to that of TALENs. We also find that off-target mutagenesis seems to be negligible, and therefore, CRISPR/Cas may be a useful system for creating genome modifications in this important model organism.

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