Aptamer-modified Magnetic Nanoprobe for Molecular MR Imaging of VEGFR2 on Angiogenic Vasculature

Overview

Journal Nanoscale Res Lett

Publisher Springer

Specialty Biotechnology

Date 2013 Sep 27

PMID 24066922

Citations 21

Authors

Bongjune Kim

Jaemoon Yang

Myeonghwan Hwang

Jihye Choi

Hyun-Ouk Kim

Eunji Jang

Jung Hwan Lee

Sung-Ho Ryu

Jin-Suck Suh

Yong-Min Huh

Seungjoo Haam

Affiliations

Soon will be listed here.

Abstract

Nucleic acid-based aptamers have been developed for the specific delivery of diagnostic nanoprobes. Here, we introduce a new class of smart imaging nanoprobe, which is based on hybridization of a magnetic nanocrystal with a specific aptamer for specific detection of the angiogenic vasculature of glioblastoma via magnetic resonance (MR) imaging. The magnetic nanocrystal imaging core was synthesized using the thermal decomposition method and enveloped by carboxyl polysorbate 80 for water solubilization and conjugation of the targeting moiety. Subsequently, the surface of the carboxylated magnetic nanocrystal was modified with amine-functionalized aptamers that specifically bind to the vascular growth factor receptor 2 (VEGFR2) that is overexpressed on angiogenic vessels. To assess the targeted imaging potential of the aptamer-conjugated magnetic nanocrystal for VEGFR2 markers, the magnetic properties and MR imaging sensitivity were investigated using the orthotopic glioblastoma mouse model. In in vivo tests, the aptamer-conjugated magnetic nanocrystal effectively targeted VEGFR2 and demonstrated excellent MR imaging sensitivity with no cytotoxicity.

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