Solution Properties and in Vitro Anti-tumor Activities of Polysaccharides from Longan Pulp

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2013 Sep 21

PMID 24051475

Citations 6

Authors

Yang Yi

Fei Huang

Ming-Wei Zhang

Rui-Fen Zhang

Yuan-Yuan Deng

Zhen-Cheng Wei

Jing-Ren He

Affiliations

Soon will be listed here.

Abstract

The solution properties of four fractions (LPI-IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration <S²>(z)(½) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <S²>(z)(½) = k M(w)(v) were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [η] on M(w) was established to be [η] = 5.3 × 10⁻²M(w)⁰·⁶¹ (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII-IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50-400 µg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides.

Citing Articles

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Effects of a Lysine-Involved Maillard Reaction on the Structure and In Vitro Activities of Polysaccharides from Longan Pulp.

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