Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-protein Complexes of Longan Pulp

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2011 Jul 23

PMID 21778950

Citations 17

Authors

Yang Yi

Sen Tai Liao

Ming Wei Zhang

John Shi

Rui Fen Zhang

Yuan Yuan Deng

Zhen Cheng Wei

Affiliations

Soon will be listed here.

Abstract

Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally composed of glucose, arabinose and mannose. LP3 displayed the maximal moisture absorption, and the thermal stability of LP2 was obviously higher than that of LP1 and LP3. All of them showed the characteristic polysaccharide and protein bands in the Fourier Transform Infrared (FTIR) spectrum. For a certain dose, all the fractions could significantly stimulate splenocyte proliferation, macrophage phagocytosis against neutral red, and NK cell cytotoxicity against YAC-1 lymphoma cell (P < 0.05). The results demonstrated that the polysaccharide-protein complexes of longan pulp have medical potential as immunotherapeutic adjuvants due to their immunomodulatory activities.

Citing Articles

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References

BLUMENKRANTZ N, ASBOE-HANSEN G . New method for quantitative determination of uronic acids. Anal Biochem. 1973; 54(2):484-9. DOI: 10.1016/0003-2697(73)90377-1. View

Tao Y, Zhang Y, Zhang L . Chemical modification and antitumor activities of two polysaccharide-protein complexes from Pleurotus tuber-regium. Int J Biol Macromol. 2009; 45(2):109-15. DOI: 10.1016/j.ijbiomac.2009.04.010. View

Chen H, Tsai Y, Lin S, Lin C, Khoo K, Lin C . Studies on the immuno-modulating and anti-tumor activities of Ganoderma lucidum (Reishi) polysaccharides. Bioorg Med Chem. 2004; 12(21):5595-601. DOI: 10.1016/j.bmc.2004.08.003. View

Wang P, Wang S, Lin S, Lin Z . [Comparison of the immunomodulatory effects of spore polysaccharides and broken spore polysaccharides isolated from Ganoderma lucidum on murine splenic lymphocytes and peritoneal macrophages in vitro]. Beijing Da Xue Xue Bao Yi Xue Ban. 2005; 37(6):569-74. View

Chen C, Yang X, Li X, Mi K, Yang Q . [A comparison study on the physical/chemical properties and immunomodulatory activities of mycelial polysaccharide extracts from five Ganoderma species]. Wei Sheng Wu Xue Bao. 2007; 47(4):628-33. View

Zhong K, Wang Q, He Y, He X . Evaluation of radicals scavenging, immunity-modulatory and antitumor activities of longan polysaccharides with ultrasonic extraction on in S180 tumor mice models. Int J Biol Macromol. 2010; 47(3):356-60. DOI: 10.1016/j.ijbiomac.2010.05.022. View

Leung M, Liu C, Zhu L, Hui Y, Yu B, Fung K . Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw.) Berg. Glycobiology. 2004; 14(6):501-10. DOI: 10.1093/glycob/cwh050. View

Gan L, Zhang S, Liu Q, Xu H . A polysaccharide-protein complex from Lycium barbarum upregulates cytokine expression in human peripheral blood mononuclear cells. Eur J Pharmacol. 2003; 471(3):217-22. DOI: 10.1016/s0014-2999(03)01827-2. View

Li X, Jiao L, Zhang X, Tian W, Chen S, Zhang L . Anti-tumor and immunomodulating activities of proteoglycans from mycelium of Phellinus nigricans and culture medium. Int Immunopharmacol. 2008; 8(6):909-15. DOI: 10.1016/j.intimp.2008.02.008. View

10.

Zheng R, Jie S, Hanchuan D, Moucheng W . Characterization and immunomodulating activities of polysaccharide from Lentinus edodes. Int Immunopharmacol. 2005; 5(5):811-20. DOI: 10.1016/j.intimp.2004.11.011. View

11.

Lin K, Kao Y, Kuo H, Yang W, Chou A, Lin H . Reishi polysaccharides induce immunoglobulin production through the TLR4/TLR2-mediated induction of transcription factor Blimp-1. J Biol Chem. 2006; 281(34):24111-23. DOI: 10.1074/jbc.M601106200. View

12.

capek P, Matulova M, Combourieu B . The extracellular proteoglycan produced by Rhodella grisea. Int J Biol Macromol. 2008; 43(4):390-3. DOI: 10.1016/j.ijbiomac.2008.07.015. View

13.

Park S, Park D, Kim D, Lee S, Yoon B, Jung W . The memory-enhancing effects of Euphoria longan fruit extract in mice. J Ethnopharmacol. 2010; 128(1):160-5. DOI: 10.1016/j.jep.2010.01.001. View

14.

Zhang J, Tang Q, Reutter W, Fan H . Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum. Life Sci. 2002; 71(6):623-38. DOI: 10.1016/s0024-3205(02)01690-9. View

15.

Liu J, Yang F, Ye L, Yang X, Timani K, Zheng Y . Possible mode of action of antiherpetic activities of a proteoglycan isolated from the mycelia of Ganoderma lucidum in vitro. J Ethnopharmacol. 2004; 95(2-3):265-72. DOI: 10.1016/j.jep.2004.07.010. View

16.

Chen Z, Tan B, Chan S . Activation of T lymphocytes by polysaccharide-protein complex from Lycium barbarum L. Int Immunopharmacol. 2008; 8(12):1663-71. DOI: 10.1016/j.intimp.2008.07.019. View

17.

Kim G, Lee M, Lee H, Moon D, Lee C, Jin C . Effect of water-soluble proteoglycan isolated from Agaricus blazei on the maturation of murine bone marrow-derived dendritic cells. Int Immunopharmacol. 2005; 5(10):1523-32. DOI: 10.1016/j.intimp.2005.02.018. View

18.

Yang B, Zhao M, Jiang Y . Optimization of tyrosinase inhibition activity of ultrasonic-extracted polysaccharides from longan fruit pericarp. Food Chem. 2015; 110(2):294-300. DOI: 10.1016/j.foodchem.2008.01.067. View

19.

Lo T, Jiang Y, Chao A, Chang C . Use of statistical methods to find the polysaccharide structural characteristics and the relationships between monosaccharide composition ratio and macrophage stimulatory activity of regionally different strains of Lentinula edodes. Anal Chim Acta. 2007; 584(1):50-6. DOI: 10.1016/j.aca.2006.10.051. View

20.

Guo L, Xie J, Ruan Y, Zhou L, Zhu H, Yun X . Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum. Int Immunopharmacol. 2009; 9(10):1175-82. DOI: 10.1016/j.intimp.2009.06.005. View