In Vivo Two-photon Imaging of the Mouse Retina

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2013 Sep 7

PMID 24009992

Citations 38

Authors

Robin Sharma

Lu Yin

Ying Geng

William H Merigan

Grazyna Palczewska

Krzysztof Palczewski

David R Williams

Jennifer J Hunter

Affiliations

Soon will be listed here.

Abstract

Though in vivo two-photon imaging has been demonstrated in non-human primates, improvements in the signal-to-noise ratio (SNR) would greatly improve its scientific utility. In this study, extrinsic fluorophores, expressed in otherwise transparent retinal ganglion cells, were imaged in the living mouse eye using a two-photon fluorescence adaptive optics scanning laser ophthalmoscope. We recorded two orders of magnitude greater signal levels from extrinsically labeled cells relative to previous work done in two-photon autofluorescence imaging of primates. Features as small as single dendrites in various layers of the retina could be resolved and predictions are made about the feasibility of measuring functional response from cells. In the future, two-photon imaging in the intact eye may allow us to monitor the function of retinal cell classes with infrared light that minimally excites the visual response.

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