Practical Innovations for High-throughput Amplicon Sequencing

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2013 Sep 3

PMID 23995388

Citations 377

Authors

Derek S Lundberg

Scott Yourstone

Piotr Mieczkowski

Corbin D Jones

Jeffery L Dangl

Affiliations

Soon will be listed here.

Abstract

We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide. PCR clamps block amplification of contaminating sequences from a eukaryotic host, thereby substantially enriching microbial sequences without introducing bias.

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