Validation of the Dynamic Direct Exposure Method for Toxicity Testing of Diesel Exhaust in Vitro

Overview

Journal ISRN Toxicol

Specialty Pharmacology

Date 2013 Aug 30

PMID 23986878

Citations 3

Authors

Lucky Joeng

Amanda Hayes

Shahnaz Bakand

Affiliations

Soon will be listed here.

Abstract

Diesel exhaust emission is a major health concern because of the complex nature of its gaseous content (e.g., NO2, NO, CO, and CO2) and high concentration of particulate matter (PM) less than 2.5 μ m which allows for deeper penetration into the human pulmonary system upon inhalation. The aim of this research was to elucidate the potential toxic effects of diesel exhaust on a human pulmonary-based cellular system. Validation of a dynamic direct exposure method for both laboratory (230 hp Volvo truck engine) and field (Volkswagen Passat passenger car) diesel engines, at idle mode, was implemented. Human pulmonary type II epithelial cells (A549) grown on porous membranes were exposed to unmodified diesel exhaust at a low flow rate (37.5 mL/min). In parallel, diesel emission sampling was also conducted using real-time air monitoring techniques. Induced cellular effects were assessed using a range of in vitro cytotoxicity assays (MTS, ATP, and NRU). Reduction of cell viability was observed in a time-dependent manner following 30-60 mins of exposure with NRU as the most sensitive assay. The results suggest that the dynamic direct exposure method has the potential to be implemented for both laboratory- and field-based in vitro toxicity studies of diesel exhaust emissions.

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References

Tang M, Li Q, Xiao L, Li Y, Jensen J, Liou T . Toxicity effects of short term diesel exhaust particles exposure to human small airway epithelial cells (SAECs) and human lung carcinoma epithelial cells (A549). Toxicol Lett. 2012; 215(3):181-92. PMC: 7920584. DOI: 10.1016/j.toxlet.2012.10.016. View

Lestari F, Hayes A, Green A, Chattopadhyay G . In vitro cytotoxicity and morphological assessment of smoke from polymer combustion in human lung derived cells (A549). Int J Hyg Environ Health. 2012; 215(3):320-32. DOI: 10.1016/j.ijheh.2011.12.006. View

Goodwin C, Holt S, Downes S, Marshall N . Microculture tetrazolium assays: a comparison between two new tetrazolium salts, XTT and MTS. J Immunol Methods. 1995; 179(1):95-103. DOI: 10.1016/0022-1759(94)00277-4. View

Hirota R, Akimaru K, Nakamura H . In vitro toxicity evaluation of diesel exhaust particles on human eosinophilic cell. Toxicol In Vitro. 2008; 22(4):988-94. DOI: 10.1016/j.tiv.2008.02.004. View

Pope 3rd C, Burnett R, Thun M, Calle E, Krewski D, Ito K . Lung cancer, cardiopulmonary mortality, and long-term exposure to fine particulate air pollution. JAMA. 2002; 287(9):1132-41. PMC: 4037163. DOI: 10.1001/jama.287.9.1132. View

Lestari F, Hayes A, Green A, Markovic B . In vitro cytotoxicity of selected chemicals commonly produced during fire combustion using human cell lines. Toxicol In Vitro. 2005; 19(5):653-63. DOI: 10.1016/j.tiv.2005.03.002. View

Husoy T, Syversen T, Jenssen J . Comparisons of four in vitro cytotoxicity tests: The MTT assay, NR assay, uridine incorporation and protein measurements. Toxicol In Vitro. 2010; 7(2):149-54. DOI: 10.1016/0887-2333(93)90125-o. View

Castano A, Gomez-Lechon M . Comparison of basal cytotoxicity data between mammalian and fish cell lines: a literature survey. Toxicol In Vitro. 2005; 19(5):695-705. DOI: 10.1016/j.tiv.2005.04.002. View

Potera C . More human, more humane: a new approach for testing airborne pollutants. Environ Health Perspect. 2007; 115(3):A148-51. PMC: 1849917. DOI: 10.1289/ehp.115-a148. View

10.

Risom L, Moller P, Loft S . Oxidative stress-induced DNA damage by particulate air pollution. Mutat Res. 2005; 592(1-2):119-37. DOI: 10.1016/j.mrfmmm.2005.06.012. View

11.

Ohtoshi T, Takizawa H, Okazaki H, Kawasaki S, Takeuchi N, Ohta K . Diesel exhaust particles stimulate human airway epithelial cells to produce cytokines relevant to airway inflammation in vitro. J Allergy Clin Immunol. 1998; 101(6 Pt 1):778-85. DOI: 10.1016/S0091-6749(98)70307-0. View

12.

Bakand S, Winder C, Hayes A . Comparative in vitro cytotoxicity assessment of selected gaseous compounds in human alveolar epithelial cells. Toxicol In Vitro. 2007; 21(7):1341-7. DOI: 10.1016/j.tiv.2007.04.013. View

13.

Sauer U, Vogel S, Hess A, Kolle S, Ma-Hock L, van Ravenzwaay B . In vivo-in vitro comparison of acute respiratory tract toxicity using human 3D airway epithelial models and human A549 and murine 3T3 monolayer cell systems. Toxicol In Vitro. 2012; 27(1):174-90. DOI: 10.1016/j.tiv.2012.10.007. View

14.

Cheng M, Malone B, Storey J . Monitoring cellular responses of engine-emitted particles by using a direct air-cell interface deposition technique. Chemosphere. 2003; 53(3):237-43. DOI: 10.1016/S0045-6535(03)00555-1. View

15.

Nemmar A, Hoylaerts M, Hoet P, Nemery B . Possible mechanisms of the cardiovascular effects of inhaled particles: systemic translocation and prothrombotic effects. Toxicol Lett. 2004; 149(1-3):243-53. DOI: 10.1016/j.toxlet.2003.12.061. View

16.

Borenfreund E, Puerner J . Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett. 1985; 24(2-3):119-24. DOI: 10.1016/0378-4274(85)90046-3. View

17.

Groves J, Cain J . A survey of exposure to diesel engine exhaust emissions in the workplace. Ann Occup Hyg. 2000; 44(6):435-47. View

18.

Eisenbrand G, Pool-Zobel B, Baker V, Balls M, Blaauboer B, Boobis A . Methods of in vitro toxicology. Food Chem Toxicol. 2002; 40(2-3):193-236. DOI: 10.1016/s0278-6915(01)00118-1. View

19.

Pope 3rd C, Verrier R, Lovett E, Larson A, Raizenne M, Kanner R . Heart rate variability associated with particulate air pollution. Am Heart J. 1999; 138(5 Pt 1):890-9. DOI: 10.1016/s0002-8703(99)70014-1. View

20.

Combes R . Assessing risk to humans from chemical exposure by using non-animal test data. Toxicol In Vitro. 2005; 19(7):921-4. DOI: 10.1016/j.tiv.2005.07.001. View