Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol-3-acetate Isolated from Calotropis Gigantea (Linn.) Flower

Overview

Journal Mycobiology

Date 2013 Aug 29

PMID 23983504

Citations 34

Authors

M Rowshanul Habib

M Rezaul Karim

Affiliations

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Abstract

A phytochemical study on the flowe r of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of 16~128 µg/ml. The cytotoxicity (LC50) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 µg/ml for ethyl acetate, 9.19 µg/ml for compound 1 and 15.55 µg/ml for compound 2.

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References

Habib M, Nikkon F, Rahman M, Haque M, Karim M . Isolation of stigmasterol and beta-sitosterol from methanolic extract of root bark of Calotropis gigantea (Linn). Pak J Biol Sci. 2008; 10(22):4174-6. DOI: 10.3923/pjbs.2007.4174.4176. View

Gulyas S, Szegletes Z . Functional connection between intracellular and extracellular secretion in species of Euphorbia genus. Acta Biol Hung. 1993; 44(4):433-43. View

Kiuchi F, Fukao Y, Maruyama T, Obata T, Tanaka M, Sasaki T . Cytotoxic principles of a Bangladeshi crude drug, akond mul (roots of Calotropis gigantea L.). Chem Pharm Bull (Tokyo). 1998; 46(3):528-30. DOI: 10.1248/cpb.46.528. View

Basile A, Giordano S, Lopez-Saez J, Cobianchi R . Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry. 2000; 52(8):1479-82. DOI: 10.1016/s0031-9422(99)00286-1. View

Lhinhatrakool T, Sutthivaiyakit S . 19-Nor- and 18,20-epoxy-cardenolides from the leaves of Calotropis gigantea. J Nat Prod. 2006; 69(8):1249-51. DOI: 10.1021/np060249f. View

Kitagawa I, Zhang R, Park J, Baek N, Takeda Y, Yoshikawa M . Indonesian medicinal plants. I. Chemical structures of calotroposides A and B, two new oxypregnane-oligoglycosides from the root of Calotropis gigantea (Asclepiadaceae). Chem Pharm Bull (Tokyo). 1992; 40(8):2007-13. DOI: 10.1248/cpb.40.2007. View

Pathak A, Argal A . Analgesic activity of Calotropis gigantea flower. Fitoterapia. 2006; 78(1):40-2. DOI: 10.1016/j.fitote.2006.09.023. View

Chitme H, Chandra M, Kaushik S . Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br. in experimental animals. J Pharm Pharm Sci. 2004; 7(1):70-5. View

Rao G, Mahesh Kumar P, Dhandapani V, Rama Krishna T, Hayashi T . Constituents of Cassia auriculata. Fitoterapia. 2001; 71(1):82-3. DOI: 10.1016/s0367-326x(99)00108-2. View

10.

Pari , Rao , Devakumar , Rastogi . A Novel Insect Antifeedant Nonprotein Amino Acid from Calotropis gigantea. J Nat Prod. 1998; 61(1):102-4. DOI: 10.1021/np970255z. View

11.

Chitme H, Chandra R, Kaushik S . Evaluation of antipyretic activity of Calotropis gigantea (Asclepiadaceae) in experimental animals. Phytother Res. 2005; 19(5):454-6. DOI: 10.1002/ptr.1672. View

12.

Karaman I, Sahin F, Gulluce M, Ogutcu H, Sengul M, Adiguzel A . Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol. 2003; 85(2-3):231-5. DOI: 10.1016/s0378-8741(03)00006-0. View

13.

Oie L, Hersoug L, Madsen J . Residential exposure to plasticizers and its possible role in the pathogenesis of asthma. Environ Health Perspect. 1997; 105(9):972-8. PMC: 1470354. DOI: 10.1289/ehp.97105972. View

14.

Sen S, Sahu N, Mahato S . Flavonol glycosides from Calotropis gigantea. Phytochemistry. 1992; 31(8):2919-21. DOI: 10.1016/0031-9422(92)83668-o. View

15.

Meyer B, Ferrigni N, Putnam J, Jacobsen L, Nichols D, McLaughlin J . Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982; 45(1):31-4. View

16.

Rios J, Recio M, Villar A . Screening methods for natural products with antimicrobial activity: a review of the literature. J Ethnopharmacol. 1988; 23(2-3):127-49. DOI: 10.1016/0378-8741(88)90001-3. View

17.

Mavar-Manga H, Haddad M, Pieters L, Baccelli C, Penge A, Quetin-Leclercq J . Anti-inflammatory compounds from leaves and root bark of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg. J Ethnopharmacol. 2007; 115(1):25-9. DOI: 10.1016/j.jep.2007.08.043. View

18.

Mukherjee P, Saritha G, Suresh B . Antimicrobial potential of two different Hypericum species available in India. Phytother Res. 2002; 16(7):692-5. DOI: 10.1002/ptr.1016. View

19.

Argal A, Pathak A . CNS activity of Calotropis gigantea roots. J Ethnopharmacol. 2006; 106(1):142-5. DOI: 10.1016/j.jep.2005.12.024. View

20.

Adak M, Gupta J . Evaluation of anti-inflammatory activity of Calotropis gigantea (AKANDA) in various biological system. Nepal Med Coll J. 2007; 8(3):156-61. View