Biological Control Activity of Plant Growth Promoting Rhizobacteria AY001 Against Tomato Fusarium Wilt and Bacterial Speck Diseases

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2022 Apr 23

PMID 35453817

Authors

A Yeong Heo

Young Mo Koo

Hyong Woo Choi

Affiliations

Soon will be listed here.

Abstract

Plant growth promoting rhizobacteria (PGPR) is not only enhancing plant growth, but also inducing resistance against a broad range of pathogens, thus providing effective strategies to substitute chemical products. In this study, AY001 (AY001) is isolated based on its broad-spectrum antifungal activity. AY001 not only inhibited fungal pathogen growth in dual culture and culture filtrate assays, but also showed various PGPR traits, such as nitrogen fixation, phosphate solubilization, extracellular protease production, zinc solubilization and indole-3-acetic acid (IAA) biosynthesis activities. Indeed, AY001 treatment significantly enhanced growth of tomato plants and enhanced resistance against two distinct pathogens, . f.sp. and pv. . Real-time qPCR analyses revealed that AY001 treatment induced jasmonic acid/ethylene-dependent defense-related gene expression, suggesting its Induced Systemic Resistance (ISR)-eliciting activity. Gas chromatography-mass spectrometry (GC-MS) analysis of culture filtrate of AY001 revealed production of antimicrobial compounds, including di(2-ethylhexyl) phthalate and pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl). Taken together, our newly isolated AY001 showed promising PGPR and ISR activities in tomato plants, suggesting its potential use as a biofertilizer and biocontrol agent.

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References

Gordon S, Weber R . COLORIMETRIC ESTIMATION OF INDOLEACETIC ACID. Plant Physiol. 1951; 26(1):192-5. PMC: 437633. DOI: 10.1104/pp.26.1.192. View

Choudhary D, Prakash A, Johri B . Induced systemic resistance (ISR) in plants: mechanism of action. Indian J Microbiol. 2012; 47(4):289-97. PMC: 3450033. DOI: 10.1007/s12088-007-0054-2. View

Nautiyal C . An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett. 1999; 170(1):265-70. DOI: 10.1111/j.1574-6968.1999.tb13383.x. View

Kachroo A, Fu D, Havens W, Navarre D, Kachroo P, Ghabrial S . An oleic acid-mediated pathway induces constitutive defense signaling and enhanced resistance to multiple pathogens in soybean. Mol Plant Microbe Interact. 2008; 21(5):564-75. DOI: 10.1094/MPMI-21-5-0564. View

Dimopoulou A, Theologidis I, Liebmann B, Kalantidis K, Vassilakos N, Skandalis N . Bacillus amyloliquefaciens MBI600 differentially induces tomato defense signaling pathways depending on plant part and dose of application. Sci Rep. 2019; 9(1):19120. PMC: 6910970. DOI: 10.1038/s41598-019-55645-2. View

Fasim F, Ahmed N, Parsons R, Gadd G . Solubilization of zinc salts by a bacterium isolated from the air environment of a tannery. FEMS Microbiol Lett. 2002; 213(1):1-6. DOI: 10.1111/j.1574-6968.2002.tb11277.x. View

Klessig D, Choi H, Dempsey D . Systemic Acquired Resistance and Salicylic Acid: Past, Present, and Future. Mol Plant Microbe Interact. 2018; 31(9):871-888. DOI: 10.1094/MPMI-03-18-0067-CR. View

Zaman N, Chowdhury U, Reza R, Chowdhury F, Sarker M, Hossain M . Plant growth promoting endophyte Burkholderia contaminans NZ antagonizes phytopathogen Macrophomina phaseolina through melanin synthesis and pyrrolnitrin inhibition. PLoS One. 2021; 16(9):e0257863. PMC: 8483353. DOI: 10.1371/journal.pone.0257863. View

Abro M, Sun X, Li X, Jatoi G, Guo L . Biocontrol Potential of Fungal Endophytes against f. sp. Causing Wilt in Cucumber. Plant Pathol J. 2019; 35(6):598-608. PMC: 6901257. DOI: 10.5423/PPJ.OA.05.2019.0129. View

10.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

11.

Milling A, Babujee L, Allen C . Ralstonia solanacearum extracellular polysaccharide is a specific elicitor of defense responses in wilt-resistant tomato plants. PLoS One. 2011; 6(1):e15853. PMC: 3017055. DOI: 10.1371/journal.pone.0015853. View

12.

Compant S, Nowak J, Coenye T, Clement C, Barka E . Diversity and occurrence of Burkholderia spp. in the natural environment. FEMS Microbiol Rev. 2008; 32(4):607-26. DOI: 10.1111/j.1574-6976.2008.00113.x. View

13.

Tagele S, Kim S, Lee H, Kim H, Lee Y . Effectiveness of multi-trait Burkholderia contaminans KNU17BI1 in growth promotion and management of banded leaf and sheath blight in maize seedling. Microbiol Res. 2018; 214:8-18. DOI: 10.1016/j.micres.2018.05.004. View

14.

Weller D, Raaijmakers J, Gardener B, Thomashow L . Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu Rev Phytopathol. 2002; 40:309-48. DOI: 10.1146/annurev.phyto.40.030402.110010. View

15.

Choudhary D, Johri B . Interactions of Bacillus spp. and plants--with special reference to induced systemic resistance (ISR). Microbiol Res. 2008; 164(5):493-513. DOI: 10.1016/j.micres.2008.08.007. View

16.

Coenye T, Vandamme P . Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol. 2003; 5(9):719-29. DOI: 10.1046/j.1462-2920.2003.00471.x. View

17.

Singh V, Singh H, Upadhyay R . Role of fusaric acid in the development of 'Fusarium wilt' symptoms in tomato: Physiological, biochemical and proteomic perspectives. Plant Physiol Biochem. 2017; 118:320-332. DOI: 10.1016/j.plaphy.2017.06.028. View

18.

Farmer E, Ryan C . Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors. Plant Cell. 1992; 4(2):129-134. PMC: 160114. DOI: 10.1105/tpc.4.2.129. View

19.

Dilika F, Bremner P, Meyer J . Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia. 2000; 71(4):450-2. DOI: 10.1016/s0367-326x(00)00150-7. View

20.

Gerber I, Zeidler D, Durner J, Dubery I . Early perception responses of Nicotiana tabacum cells in response to lipopolysaccharides from Burkholderia cepacia. Planta. 2003; 218(4):647-57. DOI: 10.1007/s00425-003-1142-0. View