The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2013 Aug 21

PMID 23959986

Citations 8

Authors

Shen Y Heazlewood

Brenda Williams

Melonie J Storan

Susan K Nilsson

Affiliations

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Abstract

Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

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