Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis

Overview

Journal Mycobiology

Date 2013 Aug 20

PMID 23956636

Citations 3

Authors

Soo-Jin Cho

Young Hwan Kim

Hee-Moon Park

Kwang-Soo Shin

Affiliations

Soon will be listed here.

Abstract

To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1 (+) null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, β-glucosidase (Psu1), cell surface protein, glucan 1,3-β-glucosidase (Bgl2), and exo-1,3 β-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.

Citing Articles

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