Interactome Analysis Reveals That C1QBP (complement Component 1, Q Subcomponent Binding Protein) is Associated with Cancer Cell Chemotaxis and Metastasis

Overview

Journal Mol Cell Proteomics

Specialties Biochemistry
Cell Biology
Molecular Biology

Date 2013 Aug 9

PMID 23924515

Citations 36

Authors

Xiaofang Zhang

Fei Zhang

Lin Guo

Yanping Wang

Peng Zhang

Ruirui Wang

Ning Zhang

Ruibing Chen

Affiliations

Soon will be listed here.

Abstract

The complement component 1, q subcomponent binding protein (C1QBP/p32/HABP1) is a ubiquitously expressed and multicompartmental cellular protein involved in various biological processes. In order to further understand its biological functions, we conducted proteomics analysis of its interactome in this study. An improved sample preparation and mass spectrometric identification strategy was developed combining high-speed centrifugation, formaldehyde labeling, and two-dimensional reverse-phase liquid chromatography. Using this approach, we identified 187 interacting proteins and constructed a highly connected interacting network for C1QBP. Moreover, we explored the interaction between C1QBP and protein kinase C ζ, a key regulator of cell polarity and migration. The results indicated that C1QBP regulated the activity of protein kinase C ζ and modulated EGF-induced cancer cell chemotaxis. In addition, C1QBP was required for breast cancer metastasis in a severe combined immunodeficiency mouse model. Furthermore, C1QBP was observed to be overexpressed in breast cancer tissues, and its expression level was closely linked with distant metastasis and TNM stages. In summary, C1QBP was identified as a novel regulator of cancer metastasis that may serve as a therapeutic target for breast cancer treatment.

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References

Ghosh I, Roy Chowdhury A, Rajeswari M, Datta K . Differential expression of Hyaluronic Acid Binding Protein 1 (HABP1)/P32/C1QBP during progression of epidermal carcinoma. Mol Cell Biochem. 2005; 267(1-2):133-9. DOI: 10.1023/b:mcbi.0000049362.04033.ea. View

He J, Gu D, Wu X, Reynolds K, Duan X, Yao C . Major causes of death among men and women in China. N Engl J Med. 2005; 353(11):1124-34. DOI: 10.1056/NEJMsa050467. View

Krainer A, Mayeda A, Kozak D, Binns G . Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators. Cell. 1991; 66(2):383-94. DOI: 10.1016/0092-8674(91)90627-b. View

Mann M . Functional and quantitative proteomics using SILAC. Nat Rev Mol Cell Biol. 2006; 7(12):952-8. DOI: 10.1038/nrm2067. View

Etienne-Manneville S, Hall A . Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity. Nature. 2003; 421(6924):753-6. DOI: 10.1038/nature01423. View

Ghebrehiwet B, Lim B, Peerschke E, Willis A, Reid K . Isolation, cDNA cloning, and overexpression of a 33-kD cell surface glycoprotein that binds to the globular "heads" of C1q. J Exp Med. 1994; 179(6):1809-21. PMC: 2191527. DOI: 10.1084/jem.179.6.1809. View

Chattopadhyay C, Hawke D, Kobayashi R, Maity S . Human p32, interacts with B subunit of the CCAAT-binding factor, CBF/NF-Y, and inhibits CBF-mediated transcription activation in vitro. Nucleic Acids Res. 2004; 32(12):3632-41. PMC: 484179. DOI: 10.1093/nar/gkh692. View

Gupta S, Batchu R, Datta K . Purification, partial characterization of rat kidney hyaluronic acid binding protein and its localization on the cell surface. Eur J Cell Biol. 1991; 56(1):58-67. View

Chen R, Hui L, Cape S, Wang J, Li L . Comparative Neuropeptidomic Analysis of Food Intake via a Multi-faceted Mass Spectrometric Approach. ACS Chem Neurosci. 2010; 1(3):204-214. PMC: 2847303. DOI: 10.1021/cn900028s. View

10.

Muta T, Kang D, Kitajima S, Fujiwara T, Hamasaki N . p32 protein, a splicing factor 2-associated protein, is localized in mitochondrial matrix and is functionally important in maintaining oxidative phosphorylation. J Biol Chem. 1997; 272(39):24363-70. DOI: 10.1074/jbc.272.39.24363. View

11.

Seo M, Lee W, Suk K . Identification of novel cell migration-promoting genes by a functional genetic screen. FASEB J. 2009; 24(2):464-78. DOI: 10.1096/fj.09-137562. View

12.

Estmer C, Ohrmalm C, Matthews D, Russell W, Akusjarvi G . The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. EMBO J. 1999; 18(4):1014-24. PMC: 1171193. DOI: 10.1093/emboj/18.4.1014. View

13.

Bialucha C, Ferber E, Pichaud F, Peak-Chew S, Fujita Y . p32 is a novel mammalian Lgl binding protein that enhances the activity of protein kinase Czeta and regulates cell polarity. J Cell Biol. 2007; 178(4):575-81. PMC: 2064465. DOI: 10.1083/jcb.200612022. View

14.

Tsuboi S . A complex of Wiskott-Aldrich syndrome protein with mammalian verprolins plays an important role in monocyte chemotaxis. J Immunol. 2006; 176(11):6576-85. DOI: 10.4049/jimmunol.176.11.6576. View

15.

Etienne-Manneville S, Hall A . Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCzeta. Cell. 2001; 106(4):489-98. DOI: 10.1016/s0092-8674(01)00471-8. View

16.

Roussos E, Condeelis J, Patsialou A . Chemotaxis in cancer. Nat Rev Cancer. 2011; 11(8):573-87. PMC: 4030706. DOI: 10.1038/nrc3078. View

17.

Chen R, Wang Y, Liu Y, Zhang Q, Zhang X, Zhang F . Quantitative study of the interactome of PKCζ involved in the EGF-induced tumor cell chemotaxis. J Proteome Res. 2013; 12(3):1478-86. DOI: 10.1021/pr3011292. View

18.

Zhang F, Zhang X, Li M, Chen P, Zhang B, Guo H . mTOR complex component Rictor interacts with PKCzeta and regulates cancer cell metastasis. Cancer Res. 2010; 70(22):9360-70. DOI: 10.1158/0008-5472.CAN-10-0207. View

19.

Majumdar M, Meenakshi J, Goswami S, Datta K . Hyaluronan binding protein 1 (HABP1)/C1QBP/p32 is an endogenous substrate for MAP kinase and is translocated to the nucleus upon mitogenic stimulation. Biochem Biophys Res Commun. 2002; 291(4):829-37. DOI: 10.1006/bbrc.2002.6491. View

20.

Wang T, Gu S, Ronni T, Du Y, Chen X . In vivo dual-tagging proteomic approach in studying signaling pathways in immune response. J Proteome Res. 2005; 4(3):941-9. DOI: 10.1021/pr050031z. View