Cut, Via CrebA, Transcriptionally Regulates the COPII Secretory Pathway to Direct Dendrite Development in Drosophila

Overview

Journal J Cell Sci

Specialty Cell Biology

Date 2013 Aug 2

PMID 23902691

Citations 30

Authors

Srividya Chandramouli Iyer

Eswar P Ramachandran Iyer

Ramakrishna Meduri

Myurajan Rubaharan

Aravinda Kuntimaddi

Madhu Karamsetty

Daniel N Cox

Affiliations

Soon will be listed here.

Abstract

Dendrite development is crucial in the formation of functional neural networks. Recent studies have provided insights into the involvement of secretory transport in dendritogenesis, raising the question of how the secretory pathway is controlled to direct dendritic elaboration. Here, we identify a functional link between transcriptional regulatory programs and the COPII secretory machinery in driving dendrite morphogenesis in Drosophila dendritic arborization (da) sensory neurons. MARCM analyses and gain-of-function studies reveal cell-autonomous requirements for the COPII coat protein Sec31 in mediating da neuron dendritic homeostasis. We demonstrate that the homeodomain protein Cut transcriptionally regulates Sec31 in addition to other components of COPII secretory transport, to promote dendrite elaboration, accompanied by increased satellite secretory endoplasmic reticulum (ER) and Golgi outposts primarily localized to dendritic branch points. We further establish a novel functional role for the transcription factor CrebA in regulating dendrite development and show that Cut initiates a gene expression cascade through CrebA that coordinately affects the COPII machinery to mediate dendritic morphology.

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