Anti-inflammatory Activity of Baicalein in LPS-stimulated RAW264.7 Macrophages Via Estrogen Receptor and NF-κB-dependent Pathways

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2013 Jul 30

PMID 23892998

Citations 39

Authors

Guan-Wei Fan

Yuan Zhang

Xiaorui Jiang

Yan Zhu

Bingyao Wang

Lina Su

Wenjie Cao

Han Zhang

Xiumei Gao

Affiliations

Soon will be listed here.

Abstract

Baicalein has been used for many years as a popular antiviral and antibacterial in China. Recent investigations revealed that baicalein also has anti-inflammatory activities. Our results indicated that baicalein increases ERE-luciferase activity in an estrogen receptor (ER)-dependent manner when either ERα or ERβ were coexpressed in Hela cells. This study examined whether baicalein exerts an anti-inflammatory effect in RAW264.7 cells through an estrogen receptor-dependent pathway and through regulation of NF-ĸB activation. In lipopolysaccharide (LPS)-induced RAW264.7 cells, baicalein exerts anti-inflammatory effects by inhibiting iNOS, COX-2, and TNF-α mRNA expression; NO production; as well as inflammatory cytokine (IL-1β, PGE2, and TNF-α) production through an ER-dependent pathway. These effects are accompanied with the inhibition of the transcription factor NF-ĸB activation and IκBα phosphorylation. We therefore conclude that baicalein inhibits LPS-induced inflammatory cytokine production via regulation of the NF-ĸB pathway and estrogen-like activity, suggesting that it may be useful for preventing inflammation-related diseases.

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