The Molecular Diversity of α-gliadin Genes in the Tribe Triticeae

Overview

Journal Genetica

Specialties Cell Biology
Genetics

Date 2013 Jul 30

PMID 23892918

Citations 6

Authors

Peng-Fei Qi

Qing Chen

Therese Ouellet

Zhao Wang

Cheng-Xing Le

Yu-Ming Wei

Xiu-Jin Lan

You-Liang Zheng

Affiliations

Soon will be listed here.

Abstract

Many of the unique properties of wheat flour are derived from seed storage proteins such as the α-gliadins. In this study these α-gliadin genes from diploid Triticeae species were systemically characterized, and divided into 3 classes according to the distinct organization of their protein domains. Our analyses indicated that these α-gliadins varied in the number of cysteine residues they contained. Most of the α-gliadin genes were grouped according to their genomic origins within the phylogenetic tree. As expected, sequence alignments suggested that the repetitive domain and the two polyglutamine regions were responsible for length variations of α-gliadins as were the insertion/deletion of structural domains within the three different classes (I, II, and III) of α-gliadins. A screening of celiac disease toxic epitopes indicated that the α-gliadins of the class II, derived from the Ns genome, contain no epitope, and that some other genomes contain much fewer epitopes than the A, S(B) and D genomes of wheat. Our results suggest that the observed genetic differences in α-gliadins of Triticeae might indicate their use as a fertile ground for the breeding of less CD-toxic wheat varieties.

Citing Articles

Multiple Wheat Genomes Reveal Novel Sublocus Location and Variation of Celiac Disease Epitopes in Duplicated α-Gliadin Genes.

Halstead-Nussloch G, Tanaka T, Copetti D, Paape T, Kobayashi F, Hatakeyama M Front Plant Sci. 2021; 12:715985.

PMID: 34539709 PMC: 8446623. DOI: 10.3389/fpls.2021.715985.

Mapping Coeliac Toxic Motifs in the Prolamin Seed Storage Proteins of Barley, Rye, and Oats Using a Curated Sequence Database.

Daly M, Bromilow S, Nitride C, Shewry P, Gethings L, Mills E Front Nutr. 2020; 7:87.

PMID: 32766270 PMC: 7379453. DOI: 10.3389/fnut.2020.00087.

Celiac disease: from etiological factors to evolving diagnostic approaches.

Kaur A, Shimoni O, Wallach M J Gastroenterol. 2017; 52(9):1001-1012.

PMID: 28631048 DOI: 10.1007/s00535-017-1357-7.

Allelic variations of α-gliadin genes from species of Aegilops section Sitopsis and insights into evolution of α-gliadin multigene family among Triticum and Aegilops.

Huang Z, Long H, Wei Y, Yan Z, Zheng Y Genetica. 2016; 144(2):213-22.

PMID: 26940567 DOI: 10.1007/s10709-016-9891-4.

Genome-, Transcriptome- and Proteome-Wide Analyses of the Gliadin Gene Families in Triticum urartu.

Zhang Y, Luo G, Liu D, Wang D, Yang W, Sun J PLoS One. 2015; 10(7):e0131559.

PMID: 26132381 PMC: 4489009. DOI: 10.1371/journal.pone.0131559.

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