Studies on the Assembly Characteristics of Large Subunit Ribosomal Proteins in S. Cerevisae

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Jul 23

PMID 23874617

Citations 46

Authors

Uli Ohmayer

Michael Gamalinda

Martina Sauert

Julius Ossowski

Gisela Poll

Jan Linnemann

Thomas Hierlmeier

Jorge Perez-Fernandez

Beril Kumcuoglu

Isabelle Leger-Silvestre

Marlene Faubladier

Joachim Griesenbeck

John Woolford

Herbert Tschochner

Philipp Milkereit

Affiliations

Soon will be listed here.

Abstract

During the assembly process of ribosomal subunits, their structural components, the ribosomal RNAs (rRNAs) and the ribosomal proteins (r-proteins) have to join together in a highly dynamic and defined manner to enable the efficient formation of functional ribosomes. In this work, the assembly of large ribosomal subunit (LSU) r-proteins from the eukaryote S. cerevisiae was systematically investigated. Groups of LSU r-proteins with specific assembly characteristics were detected by comparing the protein composition of affinity purified early, middle, late or mature LSU (precursor) particles by semi-quantitative mass spectrometry. The impact of yeast LSU r-proteins rpL25, rpL2, rpL43, and rpL21 on the composition of intermediate to late nuclear LSU precursors was analyzed in more detail. Effects of these proteins on the assembly states of other r-proteins and on the transient LSU precursor association of several ribosome biogenesis factors, including Nog2, Rsa4 and Nop53, are discussed.

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