Superovulation Induces Defective Methylation in Line-1 Retrotransposon Elements in Blastocyst

Overview

Journal Reprod Biol Endocrinol

Publisher Biomed Central

Specialties Endocrinology
Reproductive Medicine

Date 2013 Jul 20

PMID 23866265

Citations 14

Authors

Xing-Wei Liang

Xiang-Shun Cui

Shao-Chen Sun

Yong-Xun Jin

Young Tae Heo

Suk Namgoong

Nam-Hyung Kim

Affiliations

Soon will be listed here.

Abstract

Background: Series of epigenetic events happen during preimplantation development. Therefore assistant reproduction techniques (ART) have the potential to disrupt epigenetic regulation during embryo development. The purpose of this study was to investigate whether defects in methylation patterns in blastocyst due to superovulation originate from abnormal expression of Dnmts.

Methods: Low- (6 IU) and high- (10 IU) dosage of PMSG was used to stimulate the female mice. The metaphase II(MII) oocytes, zygotes and blastocyst stage embryos were collected. Global methylation and methylation at H3K9 in zygote, and methylation at repeated sequence Line 1 and IAP in blastocysts were assayed. In addition, expression of Dnmts was examined in oocytes and zygotes.

Results: Global DNA methylation and methylation at H3K9 in zygotes derived from females after low- or high-dosage hormone treatment were unaltered compared to that in controls. Moreover, DNA methylation at IAP in blastocysts was also unaffected, regardless of hormone dosage. In contrast, methylation at Line1 decreased when high-dose hormone was administered. Unexpectedly, expression of Dnmt3a, Dnmt3b, Dnmt3L as well as maintenance Dnmt1o in oocytes and zygotes was not disrupted.

Conclusions: The results suggest that defects in embryonic methylation patterns do not originate from the disruption of Dnmt expression.

Citing Articles

Participation of preovulatory follicles in the activation of primordial follicles in mouse ovaries.

Zhang J, Xia W, Zhou J, Qin S, Lin L, Zhao T Int J Biol Sci. 2024; 20(10):3863-3880.

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Genome-wide assessment of DNA methylation alterations induced by superovulation, sexual immaturity and in vitro follicle growth in mouse blastocysts.

Saucedo-Cuevas L, Ivanova E, Herta A, Krueger F, Billooye K, Smitz J Clin Epigenetics. 2023; 15(1):9.

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Repeated Superovulation Accelerates Primordial Follicle Activation and Atresia.

Wang Q, Zhao S, He J, Zhao H, Gu B, Xie J Cells. 2023; 12(1).

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Effect of Superovulation Treatment on Oocyte's DNA Methylation.

Lopes J, Ivanova E, Ruiz S, Andrews S, Kelsey G, Coy P Int J Mol Sci. 2022; 23(24).

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Editorial: Intra- and extra-environment and reproduction.

Ge Z, Klinger F, Taketo T Front Cell Dev Biol. 2022; 10:1020470.

PMID: 36407099 PMC: 9672936. DOI: 10.3389/fcell.2022.1020470.

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