Plasma Concentration of Cis9trans11 CLA in Males and Females is Influenced by SCD1 Genetic Variations and Hormonal Contraceptives: a Cross-sectional Study

Overview

Journal Nutr Metab (Lond)

Publisher Biomed Central

Date 2013 Jul 20

PMID 23866021

Citations 8

Authors

Salma A Abdelmagid

Shannon E Clarke

Jeremy Wong

Kaitlin Roke

Daiva Nielsen

Alaa Badawi

Ahmed El-Sohemy

David M Mutch

David Wl Ma

Affiliations

Soon will be listed here.

Abstract

Background: The conjugated linoleic acid isomer cis9trans11 CLA can be endogenously synthesized from trans vaccenic acid (C18:1 t11) via desaturation at the delta 9 position catalyzed by the stearoyl-CoA desaturase 1 (SCD1), also known as delta-9 desaturase (D9D). Diet, hormonal regulation of gene expression and single nucleotide polymorphisms (SNPs) have been implicated in altering circulating levels of fatty acids. Hormonal contraceptives (HC) have also been shown to influence levels of some fatty acids. SNPs in SCD1 have been associated with altered levels of palmitoleic and oleic acids; however, associations between SCD1 SNPs and D9D desaturation index have not been previously examined in relation to CLA. Herein, we investigated the effects of sex and HC use on circulating concentrations of c9t11 CLA and D9D desaturation index. Furthermore, we determined the effects of ten SCD1 SNPs on D9D desaturation indices estimated by product to precursor ratio of c9t11 CLA to C18:1 t11.

Methods: PLASMA SAMPLES WERE COLLECTED FROM SUBJECTS (CAUCASIAN MALES: n = 113; Caucasian females: n = 298; Asian males: n = 98; Asian females: n = 277) from the Toronto Nutrigenomics and Health Study. Circulating fatty acids levels were measured by gas chromatography.

Results: Results show that circulating c9t11 CLA concentrations are significantly higher in females than males and they are further elevated in females using HC. In addition, a significant sex- and ethnic-specific association was found between SCD1 SNP rs10883463 (p = 0.0014) and altered D9D activity in Caucasian males.

Conclusion: Findings from the present study identify SCD1 SNPs and hormonal contraceptives as factors altering endogenous c9t11 CLA levels in a sex- and ethnic-specific manner.

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References

Kraft J, Hanske L, Mockel P, Zimmermann S, Hartl A, Kramer J . The conversion efficiency of trans-11 and trans-12 18:1 by Delta9-desaturation differs in rats. J Nutr. 2006; 136(5):1209-14. DOI: 10.1093/jn/136.5.1209. View

Liu X, Strable M, Ntambi J . Stearoyl CoA desaturase 1: role in cellular inflammation and stress. Adv Nutr. 2012; 2(1):15-22. PMC: 3042787. DOI: 10.3945/an.110.000125. View

Herbel B, McGuire M, McGuire M, Shultz T . Safflower oil consumption does not increase plasma conjugated linoleic acid concentrations in humans. Am J Clin Nutr. 1998; 67(2):332-7. DOI: 10.1093/ajcn/67.2.332. View

Warensjo E, Ingelsson E, Lundmark P, Lannfelt L, Syvanen A, Vessby B . Polymorphisms in the SCD1 gene: associations with body fat distribution and insulin sensitivity. Obesity (Silver Spring). 2007; 15(7):1732-40. DOI: 10.1038/oby.2007.206. View

Kritchevsky D, Tepper S, Wright S, Czarnecki S, Wilson T, Nicolosi R . Conjugated linoleic acid isomer effects in atherosclerosis: growth and regression of lesions. Lipids. 2004; 39(7):611-6. DOI: 10.1007/s11745-004-1273-8. View

Merino D, Johnston H, Clarke S, Roke K, Nielsen D, Badawi A . Polymorphisms in FADS1 and FADS2 alter desaturase activity in young Caucasian and Asian adults. Mol Genet Metab. 2011; 103(2):171-8. DOI: 10.1016/j.ymgme.2011.02.012. View

Pariza M, Hargraves W . A beef-derived mutagenesis modulator inhibits initiation of mouse epidermal tumors by 7,12-dimethylbenz[a]anthracene. Carcinogenesis. 1985; 6(4):591-3. DOI: 10.1093/carcin/6.4.591. View

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

Dilzer A, Park Y . Implication of conjugated linoleic acid (CLA) in human health. Crit Rev Food Sci Nutr. 2012; 52(6):488-513. DOI: 10.1080/10408398.2010.501409. View

10.

Lee I, Paffenbarger Jr R . Physical activity and stroke incidence: the Harvard Alumni Health Study. Stroke. 1998; 29(10):2049-54. DOI: 10.1161/01.str.29.10.2049. View

11.

Kay J, Mackle T, Auldist M, Thomson N, Bauman D . Endogenous synthesis of cis-9, trans-11 conjugated linoleic acid in dairy cows fed fresh pasture. J Dairy Sci. 2004; 87(2):369-78. DOI: 10.3168/jds.S0022-0302(04)73176-8. View

12.

Iversen S, Cawood P, Dormandy T . A method for the measurement of a diene-conjugated derivative of linoleic acid, 18:2(9,11), in serum phospholipid, and possible origins. Ann Clin Biochem. 1985; 22 ( Pt 2):137-40. DOI: 10.1177/000456328502200204. View

13.

Fontaine-Bisson B, Wolever T, Connelly P, Corey P, El-Sohemy A . NF-kappaB -94Ins/Del ATTG polymorphism modifies the association between dietary polyunsaturated fatty acids and HDL-cholesterol in two distinct populations. Atherosclerosis. 2008; 204(2):465-70. DOI: 10.1016/j.atherosclerosis.2008.10.037. View

14.

Belury M . Inhibition of carcinogenesis by conjugated linoleic acid: potential mechanisms of action. J Nutr. 2002; 132(10):2995-8. DOI: 10.1093/jn/131.10.2995. View

15.

Stender S, Astrup A, Dyerberg J . Ruminant and industrially produced trans fatty acids: health aspects. Food Nutr Res. 2008; 52. PMC: 2596737. DOI: 10.3402/fnr.v52i0.1651. View

16.

Huot P, Sarkar B, Ma D . Conjugated linoleic acid alters caveolae phospholipid fatty acid composition and decreases caveolin-1 expression in MCF-7 breast cancer cells. Nutr Res. 2010; 30(3):179-85. DOI: 10.1016/j.nutres.2010.02.003. View

17.

Blewett H, Gerdung C, Ruth M, Proctor S, Field C . Vaccenic acid favourably alters immune function in obese JCR:LA-cp rats. Br J Nutr. 2009; 102(4):526-36. DOI: 10.1017/S0007114509231722. View

18.

Baylin A, Kim M, Donovan-Palmer A, Siles X, Dougherty L, Tocco P . Fasting whole blood as a biomarker of essential fatty acid intake in epidemiologic studies: comparison with adipose tissue and plasma. Am J Epidemiol. 2005; 162(4):373-81. DOI: 10.1093/aje/kwi213. View

19.

Mauvoisin D, Mounier C . Hormonal and nutritional regulation of SCD1 gene expression. Biochimie. 2010; 93(1):78-86. DOI: 10.1016/j.biochi.2010.08.001. View

20.

Stryjecki C, Roke K, Clarke S, Nielsen D, Badawi A, El-Sohemy A . Enzymatic activity and genetic variation in SCD1 modulate the relationship between fatty acids and inflammation. Mol Genet Metab. 2012; 105(3):421-7. DOI: 10.1016/j.ymgme.2011.12.003. View