The Arf GAP SMAP2 is Necessary for Organized Vesicle Budding from the Trans-Golgi Network and Subsequent Acrosome Formation in Spermiogenesis

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2013 Jul 19

PMID 23864717

Citations 17

Authors

Tomo Funaki

Shunsuke Kon

Kenji Tanabe

Waka Natsume

Sayaka Sato

Tadafumi Shimizu

Naomi Yoshida

Won Fen Wong

Atsuo Ogura

Takehiko Ogawa

Kimiko Inoue

Narumi Ogonuki

Hiromi Miki

Keiji Mochida

Keisuke Endoh

Kentarou Yomogida

Manabu Fukumoto

Reiko Horai

Yoichiro Iwakura

Chizuru Ito

Kiyotaka Toshimori

Toshio Watanabe

Masanobu Satake

Affiliations

Soon will be listed here.

Abstract

The trans-Golgi network (TGN) functions as a hub organelle in the exocytosis of clathrin-coated membrane vesicles, and SMAP2 is an Arf GTPase-activating protein that binds to both clathrin and the clathrin assembly protein (CALM). In the present study, SMAP2 is detected on the TGN in the pachytene spermatocyte to the round spermatid stages of spermatogenesis. Gene targeting reveals that SMAP2-deficient male mice are healthy and survive to adulthood but are infertile and exhibit globozoospermia. In SMAP2-deficient spermatids, the diameter of proacrosomal vesicles budding from TGN increases, TGN structures are distorted, acrosome formation is severely impaired, and reorganization of the nucleus does not proceed properly. CALM functions to regulate vesicle sizes, and this study shows that CALM is not recruited to the TGN in the absence of SMAP2. Furthermore, syntaxin2, a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, is not properly concentrated at the site of acrosome formation. Thus this study reveals a link between SMAP2 and CALM/syntaxin2 in clathrin-coated vesicle formation from the TGN and subsequent acrosome formation. SMAP2-deficient mice provide a model for globozoospermia in humans.

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