AP-1 and Clathrin Are Essential for Secretory Granule Biogenesis in Drosophila

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2011 Apr 15

PMID 21490149

Citations 55

Authors

Jason Burgess

Miluska Jauregui

Julie Tan

Janet Rollins

Sylvie Lallet

Peter A Leventis

Gabrielle L Boulianne

Henry C Chang

Roland Le Borgne

Helmut Kramer

Julie A Brill

Affiliations

Soon will be listed here.

Abstract

Regulated secretion of hormones, digestive enzymes, and other biologically active molecules requires the formation of secretory granules. Clathrin and the clathrin adaptor protein complex 1 (AP-1) are necessary for maturation of exocrine, endocrine, and neuroendocrine secretory granules. However, the initial steps of secretory granule biogenesis are only minimally understood. Powerful genetic approaches available in the fruit fly Drosophila melanogaster were used to investigate the molecular pathway for biogenesis of the mucin-containing "glue granules" that form within epithelial cells of the third-instar larval salivary gland. Clathrin and AP-1 colocalize at the trans-Golgi network (TGN) and clathrin recruitment requires AP-1. Furthermore, clathrin and AP-1 colocalize with secretory cargo at the TGN and on immature granules. Finally, loss of clathrin or AP-1 leads to a profound block in secretory granule formation. These findings establish a novel role for AP-1- and clathrin-dependent trafficking in the biogenesis of mucin-containing secretory granules.

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