NKT Cell-TCR Expression Activates Conventional T Cells in Vivo, but is Largely Dispensable for Mature NKT Cell Biology

Overview

Journal PLoS Biol

Specialty Biology

Date 2013 Jul 16

PMID 23853545

Citations 24

Authors

J Christoph Vahl

Klaus Heger

Nathalie Knies

Marco Y Hein

Louis Boon

Hideo Yagita

Bojan Polic

Marc Schmidt-Supprian

Affiliations

Soon will be listed here.

Abstract

Natural killer T (NKT) cell development depends on recognition of self-glycolipids via their semi-invariant Vα14i-TCR. However, to what extent TCR-mediated signals determine identity and function of mature NKT cells remains incompletely understood. To address this issue, we developed a mouse strain allowing conditional Vα14i-TCR expression from within the endogenous Tcrα locus. We demonstrate that naïve T cells are activated upon replacement of their endogenous TCR repertoire with Vα14i-restricted TCRs, but they do not differentiate into NKT cells. On the other hand, induced TCR ablation on mature NKT cells did not affect their lineage identity, homeostasis, or innate rapid cytokine secretion abilities. We therefore propose that peripheral NKT cells become unresponsive to and thus are independent of their autoreactive TCR.

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