Alpha-tocopherol Succinate- and AMD3100-mobilized Progenitors Mitigate Radiation Combined Injury in Mice

Overview

Journal J Radiat Res

Publisher Oxford University Press

Specialties Environmental Health
Oncology
Radiology

Date 2013 Jul 2

PMID 23814114

Citations 6

Authors

Vijay K Singh

Stephen Y Wise

Oluseyi O Fatanmi

Lindsay A Beattie

Elizabeth J Ducey

Thomas M Seed

Affiliations

Soon will be listed here.

Abstract

The purpose of this study was to elucidate the role of alpha-tocopherol succinate (TS)- and AMD3100-mobilized progenitors in mitigating combined injury associated with acute radiation exposure in combination with secondary physical wounding. CD2F1 mice were exposed to high doses of cobalt-60 gamma-radiation and then transfused intravenously with 5 million peripheral blood mononuclear cells (PBMCs) from TS- and AMD3100-injected mice after irradiation. Within 1 h after irradiation, mice were exposed to secondary wounding. Mice were observed for 30 d after irradiation and cytokine analysis was conducted by multiplex Luminex assay at various time-points after irradiation and wounding. Our results initially demonstrated that transfusion of TS-mobilized progenitors from normal mice enhanced survival of acutely irradiated mice exposed 24 h prior to transfusion to supralethal doses (11.5-12.5 Gy) of (60)Co gamma-radiation. Subsequently, comparable transfusions of TS-mobilized progenitors were shown to significantly mitigate severe combined injuries in acutely irradiated mice. TS administered 24 h before irradiation was able to protect mice against combined injury as well. Cytokine results demonstrated that wounding modulates irradiation-induced cytokines. This study further supports the conclusion that the infusion of TS-mobilized progenitor-containing PBMCs acts as a bridging therapy in radiation-combined-injury mice. We suggest that this novel bridging therapeutic approach involving the infusion of TS-mobilized hematopoietic progenitors following acute radiation exposure or combined injury might be applicable to humans.

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References

Hatse S, Princen K, Bridger G, De Clercq E, Schols D . Chemokine receptor inhibition by AMD3100 is strictly confined to CXCR4. FEBS Lett. 2002; 527(1-3):255-62. DOI: 10.1016/s0014-5793(02)03143-5. View

Pellmar T, Rockwell S . Priority list of research areas for radiological nuclear threat countermeasures. Radiat Res. 2004; 163(1):115-23. DOI: 10.1667/rr3283. View

Kulkarni S, Singh P, Ghosh S, Posarac A, Singh V . Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure. Cytokine. 2013; 62(2):278-85. DOI: 10.1016/j.cyto.2013.03.009. View

Valeriote F, Baker D . THE COMBINED EFFECTS OF THERMAL TRAUMA AND X-IRRADIATION ON EARLY MORTALITY. Radiat Res. 1964; 22:693-702. View

Singh V, Brown D, Kao T, Seed T . Preclinical development of a bridging therapy for radiation casualties. Exp Hematol. 2009; 38(1):61-70. DOI: 10.1016/j.exphem.2009.10.008. View

Nagy V . Accuracy considerations in EPR dosimetry. Appl Radiat Isot. 2000; 52(5):1039-50. DOI: 10.1016/s0969-8043(00)00052-x. View

Dinarello C . A clinical perspective of IL-1β as the gatekeeper of inflammation. Eur J Immunol. 2011; 41(5):1203-17. DOI: 10.1002/eji.201141550. View

Lindsay K, Coates P, Lorimore S, Wright E . The genetic basis of tissue responses to ionizing radiation. Br J Radiol. 2007; 80 Spec No 1:S2-6. DOI: 10.1259/bjr/60507340. View

Blumenthal R, Reising A, Leon E, Goldenberg D . Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines. Clin Cancer Res. 2002; 8(5):1301-9. View

10.

Alpen E, SHELINE G . The combined effects of thermal burns and whole body X irradiation on survival time and mortality. Ann Surg. 1954; 140(1):113-8. PMC: 1609615. DOI: 10.1097/00000658-195407000-00013. View

11.

Singh V, Christensen J, Fatanmi O, Gille D, Ducey E, Wise S . Myeloid progenitors: a radiation countermeasure that is effective when initiated days after irradiation. Radiat Res. 2012; 177(6):781-91. DOI: 10.1667/rr2894.1. View

12.

Singh V, Brown D, Kao T . Alpha-tocopherol succinate protects mice from gamma-radiation by induction of granulocyte-colony stimulating factor. Int J Radiat Biol. 2010; 86(1):12-21. DOI: 10.3109/09553000903264515. View

13.

Jin F, Zhai Q, Qiu L, Meng H, Zou D, Wang Y . Degradation of BM SDF-1 by MMP-9: the role in G-CSF-induced hematopoietic stem/progenitor cell mobilization. Bone Marrow Transplant. 2008; 42(9):581-8. DOI: 10.1038/bmt.2008.222. View

14.

Krivokrysenko V, Shakhov A, Singh V, Bone F, Kononov Y, Shyshynova I . Identification of granulocyte colony-stimulating factor and interleukin-6 as candidate biomarkers of CBLB502 efficacy as a medical radiation countermeasure. J Pharmacol Exp Ther. 2012; 343(2):497-508. PMC: 3477210. DOI: 10.1124/jpet.112.196071. View

15.

Singh V, Wise S, Singh P, Posarac A, Fatanmi O, Ducey E . Alpha-tocopherol succinate-mobilized progenitors improve intestinal integrity after whole body irradiation. Int J Radiat Biol. 2012; 89(5):334-45. DOI: 10.3109/09553002.2013.762137. View

16.

Pappu R, Ramirez-Carrozzi V, Sambandam A . The interleukin-17 cytokine family: critical players in host defence and inflammatory diseases. Immunology. 2011; 134(1):8-16. PMC: 3173690. DOI: 10.1111/j.1365-2567.2011.03465.x. View

17.

Grace M, Singh V, Rhee J, Jackson 3rd W, Kao T, Whitnall M . 5-AED enhances survival of irradiated mice in a G-CSF-dependent manner, stimulates innate immune cell function, reduces radiation-induced DNA damage and induces genes that modulate cell cycle progression and apoptosis. J Radiat Res. 2012; 53(6):840-53. PMC: 3483857. DOI: 10.1093/jrr/rrs060. View

18.

Damon L, Damon L . Mobilization of hematopoietic stem cells into the peripheral blood. Expert Rev Hematol. 2010; 2(6):717-33. DOI: 10.1586/ehm.09.54. View

19.

Singh V, Singh P, Wise S, Seed T . Mobilized progenitor cells as a bridging therapy for radiation casualties: a brief review of tocopherol succinate-based approaches. Int Immunopharmacol. 2011; 11(7):842-47. DOI: 10.1016/j.intimp.2011.01.017. View

20.

Singh V, Ducey E, Brown D, Whitnall M . A review of radiation countermeasure work ongoing at the Armed Forces Radiobiology Research Institute. Int J Radiat Biol. 2011; 88(4):296-310. DOI: 10.3109/09553002.2012.652726. View