Structure and Function of Palladin's Actin Binding Domain

Overview

Journal J Mol Biol

Publisher Elsevier

Specialties Microbiology
Molecular Biology

Date 2013 Jun 29

PMID 23806659

Citations 17

Authors

Moriah R Beck

Richard D S Dixon

Silvia M Goicoechea

Grant S Murphy

Joseph G Brungardt

Matthew T Beam

Pavan Srinath

Julie Patel

Jahan Mohiuddin

Carol A Otey

Sharon L Campbell

Affiliations

Soon will be listed here.

Abstract

Here, we report the NMR structure of the actin-binding domain contained in the cell adhesion protein palladin. Previously, we demonstrated that one of the immunoglobulin domains of palladin (Ig3) is both necessary and sufficient for direct filamentous actin binding in vitro. In this study, we identify two basic patches on opposite faces of Ig3 that are critical for actin binding and cross-linking. Sedimentation equilibrium assays indicate that the Ig3 domain of palladin does not self-associate. These combined data are consistent with an actin cross-linking mechanism that involves concurrent attachment of two actin filaments by a single palladin molecule by an electrostatic mechanism. Palladin mutations that disrupt actin binding show altered cellular distributions and morphology of actin in cells, revealing a functional requirement for the interaction between palladin and actin in vivo.

Citing Articles

Functional comparison of full-length palladin to isolated actin binding domain.

Albraiki S, Ajiboye O, Sargent R, Beck M Protein Sci. 2023; 32(5):e4638.

PMID: 37027210 PMC: 10117391. DOI: 10.1002/pro.4638.

Cytoskeletal Protein Palladin in Adult Gliomas Predicts Disease Incidence, Progression, and Prognosis.

Mayer O, Bugis J, Kozlova D, Leemann A, Mansur S, Peerutin I Cancers (Basel). 2022; 14(20).

PMID: 36291914 PMC: 9600953. DOI: 10.3390/cancers14205130.

The podocyte-specific knockout of palladin in mice with a 129 genetic background affects podocyte morphology and the expression of palladin interacting proteins.

Artelt N, Ritter A, Leitermann L, Kliewe F, Schluter R, Simm S PLoS One. 2021; 16(12):e0260878.

PMID: 34879092 PMC: 8654177. DOI: 10.1371/journal.pone.0260878.

Two Motors and One Spring: Hypothetic Roles of Non-Muscle Myosin II and Submembrane Actin-Based Cytoskeleton in Cell Volume Sensing.

Barvitenko N, Aslam M, Lawen A, Saldanha C, Skverchinskaya E, Uras G Int J Mol Sci. 2021; 22(15).

PMID: 34360739 PMC: 8347689. DOI: 10.3390/ijms22157967.

Integrated Quantitative Phosphoproteomics and Cell-based Functional Screening Reveals Specific Pathological Cardiac Hypertrophy-related Phosphorylation Sites.

Kwon H, Choi H, Park S, Park W, Kim D, Park Z Mol Cells. 2021; 44(7):500-516.

PMID: 34158421 PMC: 8334354. DOI: 10.14348/molcells.2021.4002.

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