Novel Genetic Diversity Within Anopheles Punctimacula S.l.: Phylogenetic Discrepancy Between the Barcode Cytochrome C Oxidase I (COI) Gene and the RDNA Second Internal Transcribed Spacer (ITS2)

Overview

Journal Acta Trop

Publisher Elsevier

Specialty Tropical Medicine

Date 2013 Jun 29

PMID 23806568

Citations 11

Authors

Jose R Loaiza

Marilyn E Scott

Eldredge Bermingham

Oris I Sanjur

Jose R Rovira

Larissa C Dutari

Yvonne-Marie Linton

Sara Bickersmith

Jan E Conn

Affiliations

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Abstract

Anopheles punctimacula s.l. is a regional malaria vector in parts of Central America, but its role in transmission is controversial due to its unresolved taxonomic status. Two cryptic species, An. malefactor and An. calderoni, have been previously confused with this taxon, and evidence for further genetic differentiation has been proposed. In the present study we collected and morphologically identified adult female mosquitoes of An. punctimacula s.l. from 10 localities across Panama and one in Costa Rica. DNA sequences from three molecular regions, the three prime end of the mitochondrial cytochrome c oxidase I gene (3' COI), the Barcode region in the five prime end of the COI (5' COI), and the rDNA second internal transcribed spacer (ITS2) were used to test the hypothesis of new molecular lineages within An. punctimacula s.l. Phylogenetic analyses using the 3' COI depicted six highly supported molecular lineages (A-F), none of which was An. malefactor. In contrast, phylogenetic inference with the 5' COI demonstrated paraphyly. Tree topologies based on the combined COI regions and ITS2 sequence data supported the same six lineages as the 3' COI alone. As a whole this evidence suggests that An. punctimacula s.l. comprises two geographically isolated lineages, but it is not clear whether these are true species. The phylogenetic structure of the An. punctimacula cluster as well as that of other unknown lineages (C type I vs C type II; D vs E) appears to be driven by geographic partition, because members of these assemblages did not overlap spatially. We report An. malefactor for the first time in Costa Rica, but our data do not support the presence of An. calderoni in Panama.

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References

Collins F, Paskewitz S . A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species. Insect Mol Biol. 1996; 5(1):1-9. DOI: 10.1111/j.1365-2583.1996.tb00034.x. View

Wilkerson R, Strickman D, Litwak T . Illustrated key to the female anopheline mosquitoes of Central America and Mexico. J Am Mosq Control Assoc. 1990; 6(1):7-34. View

Foley D, Wilkerson R, Cooper R, Volovsek M, Bryan J . A molecular phylogeny of Anopheles annulipes (Diptera: Culicidae) sensu lato: the most species-rich anopheline complex. Mol Phylogenet Evol. 2006; 43(1):283-97. DOI: 10.1016/j.ympev.2006.10.008. View

Bora H, Das M, Ahmed A, Sharma Y . Variations in the mitochondrial DNA markers in the Anopheles (Cellia) sundaicus population from the Andaman and Nicobar Islands, India. Acta Trop. 2009; 112(2):120-4. DOI: 10.1016/j.actatropica.2009.07.007. View

Posada D . jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008; 25(7):1253-6. DOI: 10.1093/molbev/msn083. View

Achee N, Grieco J, Rejmankova E, Andre R, Vanzie E, Polanco J . Biting patterns and seasonal densities of Anopheles mosquitoes in the Cayo District, Belize, Central America with emphasis on Anopheles darlingi. J Vector Ecol. 2006; 31(1):45-57. DOI: 10.3376/1081-1710(2006)31[45:bpasdo]2.0.co;2. View

Hebert P, Cywinska A, Ball S, deWaard J . Biological identifications through DNA barcodes. Proc Biol Sci. 2003; 270(1512):313-21. PMC: 1691236. DOI: 10.1098/rspb.2002.2218. View

Loaiza J, Scott M, Bermingham E, Sanjur O, Wilkerson R, Rovira J . Late Pleistocene environmental changes lead to unstable demography and population divergence of Anopheles albimanus in the northern Neotropics. Mol Phylogenet Evol. 2010; 57(3):1341-6. PMC: 3229172. DOI: 10.1016/j.ympev.2010.09.016. View

Li C, Wilkerson R . Intragenomic rDNA ITS2 variation in the neotropical Anopheles (Nyssorhynchus) albitarsis complex (Diptera: Culicidae). J Hered. 2006; 98(1):51-9. DOI: 10.1093/jhered/esl037. View

10.

Dusfour I, Blondeau J, Harbach R, Vythilingham I, Baimai V, Trung H . Polymerase chain reaction identification of three members of the Anopheles sundaicus (Diptera: Culicidae) complex, malaria vectors in Southeast Asia. J Med Entomol. 2007; 44(5):723-31. DOI: 10.1603/0022-2585(2007)44[723:pcriot]2.0.co;2. View

11.

Loaiza J, Scott M, Bermingham E, Rovira J, Conn J . Evidence for pleistocene population divergence and expansion of Anopheles albimanus in Southern Central America. Am J Trop Med Hyg. 2010; 82(1):156-64. PMC: 2803528. DOI: 10.4269/ajtmh.2010.09-0423. View

12.

Matson R, Rios C, Banda Chavez C, Gilman R, Florin D, Sifuentes V . Improved molecular technique for the differentiation of neotropical anopheline species. Am J Trop Med Hyg. 2008; 78(3):492-8. PMC: 2366801. View

13.

Cywinska A, Hunter F, Hebert P . Identifying Canadian mosquito species through DNA barcodes. Med Vet Entomol. 2007; 20(4):413-24. DOI: 10.1111/j.1365-2915.2006.00653.x. View

14.

Ronquist F, Huelsenbeck J . MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003; 19(12):1572-4. DOI: 10.1093/bioinformatics/btg180. View

15.

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R . DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994; 3(5):294-9. View

16.

Loaiza J, Bermingham E, Scott M, Rovira J, Conn J . Species composition and distribution of adult Anopheles (Diptera: Culicidae) in Panama. J Med Entomol. 2008; 45(5):841-51. PMC: 2874935. DOI: 10.1603/0022-2585(2008)45[841:scadoa]2.0.co;2. View

17.

Bower J, Dowton M, Cooper R, Beebe N . Intraspecific concerted evolution of the rDNA ITS1 in Anopheles farauti sensu stricto (Diptera: Culicidae) reveals recent patterns of population structure. J Mol Evol. 2008; 67(4):397-411. PMC: 9931795. DOI: 10.1007/s00239-008-9161-x. View

18.

Sallum M, Wilkerson R, FORATTINI O . Taxonomic study of species formerly identified as Anopheles mediopunctatus and resurrection of An. costai (Diptera: Culicidae). J Med Entomol. 1999; 36(3):282-300. DOI: 10.1093/jmedent/36.3.282. View

19.

Wilkerson R . Redescriptions of Anopheles punctimacula and An. malefactor (Diptera: Culicidae). J Med Entomol. 1990; 27(2):225-47. DOI: 10.1093/jmedent/27.2.225. View

20.

Zarowiecki M, Loaiza J, Conn J . Towards a new role for vector systematics in parasite control. Parasitology. 2011; 138(13):1723-9. DOI: 10.1017/S003118201100062X. View