Fluorescent Microscopy As a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2013 Jun 15

PMID 23765654

Citations 11

Authors

Rose A Willett

Irina D Pokrovskaya

Vladimir V Lupashin

Affiliations

Soon will be listed here.

Abstract

Staining of molecules such as proteins and glycoconjugates allows for an analysis of their localization within the cell and provides insight into their functional status. Glycosyltransferases, a class of enzymes which are responsible for glycosylating host proteins, are mostly localized to the Golgi apparatus, and their localization is maintained in part by a protein vesicular tethering complex, the conserved oligomeric Golgi (COG) complex. Here we detail a combination of fluorescent lectin and immuno-staining in cells depleted of COG complex subunits to examine the status of Golgi glycosyltransferases. The combination of these techniques allows for a detailed characterization of the changes in function and localization of Golgi glycosyltransferases with respect to transient COG subunit depletion.

Citing Articles

Development and Initial Characterization of Cellular Models for COG Complex-Related CDG-II Diseases.

Sumya F, Pokrovskaya I, Lupashin V Front Genet. 2021; 12:733048.

PMID: 34603392 PMC: 8484713. DOI: 10.3389/fgene.2021.733048.

Abnormal Expression and Prognosis Value of COG Complex Members in Kidney Renal Clear Cell Carcinoma (KIRC).

Zhang Y, Lai H, Tang B Dis Markers. 2021; 2021:4570235.

PMID: 34539936 PMC: 8443394. DOI: 10.1155/2021/4570235.

Golgi inCOGnito: From vesicle tethering to human disease.

DSouza Z, Taher F, Lupashin V Biochim Biophys Acta Gen Subj. 2020; 1864(11):129694.

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Maintaining order: COG complex controls Golgi trafficking, processing, and sorting.

Blackburn J, DSouza Z, Lupashin V FEBS Lett. 2019; 593(17):2466-2487.

PMID: 31381138 PMC: 6771879. DOI: 10.1002/1873-3468.13570.

Defects in COG-Mediated Golgi Trafficking Alter Endo-Lysosomal System in Human Cells.

DSouza Z, Blackburn J, Kudlyk T, Pokrovskaya I, Lupashin V Front Cell Dev Biol. 2019; 7:118.

PMID: 31334232 PMC: 6616090. DOI: 10.3389/fcell.2019.00118.

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