Transplanting P75-suppressed Bone Marrow Stromal Cells Promotes Functional Behavior in a Rat Model of Spinal Cord Injury

Overview

Journal Iran Biomed J

Specialties Biology
General Medicine

Date 2013 Jun 11

PMID 23748892

Citations 6

Authors

Houri Edalat

Zahra Hajebrahimi

Vahid Pirhajati

Mansoureh Movahedin

Mahmoud Tavallaei

Mohammad-Reza Soroush

Seyed Javad Mowla

Affiliations

Soon will be listed here.

Abstract

Background: Bone marrow stromal cells (BMSC) have been successfully employed for movement deficit recovery in spinal cord injury (SCI) rat models. One of the unsettled problems in cell transplantation is the relative high proportion of cell death, specifically after neural differentiation. According to our previous studies, p75 receptor, known as the death receptor, is only expressed in BMSC in a time window of 6-12 hours following neural induction. Moreover, we have recently reported a decreased level of apoptosis in p75-suppressed BMSC in vitro. Therefore, our objective in this research was to explore the functional effects of transplanting p75:siRNA expressing BMSC in SCI rats.

Methods: Laminectomy was performed at L1 vertebra level to expose spinal cord for contusion using weight-drop method. PBS-treated SCI rats (group one) were used as negative controls, in which cavitations were observed 10 weeks after SCI. pRNA-U6.1/Hygro- (group two, as a mock) and pRNA-U6.1/Hygro-p75 shRNA- (group three) transfected BMSC were labeled with a fluorescent dye, CM-DiI, and grafted into the lesion site 7 days after surgery. The Basso-Beattie-Bresnehan locomotor rating scale was performed weekly for 10 weeks.

Results: There was a significant difference (P≤0.05) between all groups of treated rats regarding functional recovery. Specifically, the discrepancy among p75 siRNA and mock-transfected BMSC was statistically significant. P75 siRNA BMSC also revealed a higher level of in vivo survival compared to the mock BMSC.

Conclusion: Our data suggest that genetically modified BMSC that express p75:siRNA could be a more suitable source of cells for treatment of SCI.

Citing Articles

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis.

Abbaszadeh H, Niknazar S, Darabi S, Roozbahany N, Noori-Zadeh A, Ghoreishi S Anat Cell Biol. 2018; 51(3):180-188.

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Combination of RNA Interference and Stem Cells for Treatment of Central Nervous System Diseases.

Hou X, Wang L, Wang F, Zhao X, Zhang H Genes (Basel). 2017; 8(5).

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Coadministration of Dexamethasone and Has Neuroprotective Effects in Rat Animal Model with Spinal Cord Injury.

Hosseini S, Kaka G, Joghataei M, Hooshmandi M, Sadraie S, Yaghoobi K Cell J. 2017; 19(1):102-116.

PMID: 28367421 PMC: 5241506. DOI: 10.22074/cellj.2016.4868.

Exogenous Expression of Nt-3 and TrkC Genes in Bone Marrow Stromal Cells Elevated the Survival Rate of the Cells in the Course of Neural Differentiation.

Edalat H, Hajebrahimi Z, Pirhajati V, Tavallaei M, Movahedin M, Mowla S Cell Mol Neurobiol. 2016; 37(7):1187-1194.

PMID: 27891557 PMC: 11482153. DOI: 10.1007/s10571-016-0448-y.

Assessment of Neuroprotective Properties of Melissa officinalis in Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury.

Hosseini S, Kaka G, Joghataei M, Hooshmandi M, Sadraie S, Yaghoobi K ASN Neuro. 2016; 8(6).

PMID: 27815336 PMC: 5098695. DOI: 10.1177/1759091416674833.

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