Structural Mimicry in Transcription Regulation of Human RNA Polymerase II by the DNA Helicase RECQL5

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2013 Jun 11

PMID 23748380

Citations 19

Authors

Susanne A Kassube

Martin Jinek

Jie Fang

Susan Tsutakawa

Eva Nogales

Affiliations

Soon will be listed here.

Abstract

RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the binding site for the transcription elongation factor TFIIS. Our cryo-EM structure of elongating Pol II arrested in complex with RECQL5 shows that the RECQL5 helicase domain is positioned to sterically block elongation. The crystal structure of the RECQL5 KIX domain reveals similarities with TFIIS, and binding of RECQL5 to Pol II interferes with the ability of TFIIS to promote transcriptional read-through in vitro. Together, our findings reveal a dual mode of transcriptional repression by RECQL5 that includes structural mimicry of the Pol II-TFIIS interaction.

Citing Articles

Structural insights into transcriptional regulation by the helicase RECQL5.

Ariza A, Lue N, Grob P, Kaeser B, Fang J, Kassube S bioRxiv. 2025; .

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Expression of human RECQL5 in Saccharomyces cerevisiae causes transcription defects and transcription-associated genome instability.

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