Multiparameter Flow Cytometry: Advances in High Resolution Analysis

Overview

Journal Immune Netw

Specialty Allergy & Immunology

Date 2013 May 24

PMID 23700394

Citations 29

Authors

Erika A ODonnell

David N Ernst

Ravi Hingorani

Affiliations

Soon will be listed here.

Abstract

Over the past 40 years, flow cytometry has emerged as a leading, application-rich technology that supports high-resolution characterization of individual cells which function in complex cellular networks such as the immune system. This brief overview highlights advances in multiparameter flow cytometric technologies and reagent applications for characterization and functional analysis of cells modulating the immune network. These advances significantly support high-throughput and high-content analyses and enable an integrated understanding of the cellular and molecular interactions that underlie complex biological systems.

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References

Muller S, Nebe-von-Caron G . Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities. FEMS Microbiol Rev. 2010; 34(4):554-87. DOI: 10.1111/j.1574-6976.2010.00214.x. View

Krutzik P, Crane J, Clutter M, Nolan G . High-content single-cell drug screening with phosphospecific flow cytometry. Nat Chem Biol. 2007; 4(2):132-42. DOI: 10.1038/nchembio.2007.59. View

Wlodkowic D, Khoshmanesh K, Akagi J, Williams D, Cooper J . Wormometry-on-a-chip: Innovative technologies for in situ analysis of small multicellular organisms. Cytometry A. 2011; 79(10):799-813. DOI: 10.1002/cyto.a.21070. View

Schulz K, Danna E, Krutzik P, Nolan G . Single-cell phospho-protein analysis by flow cytometry. Curr Protoc Immunol. 2012; Chapter 8:8.17.1-8.17.20. DOI: 10.1002/0471142735.im0817s96. View

Appay V, van Lier R, Sallusto F, Roederer M . Phenotype and function of human T lymphocyte subsets: consensus and issues. Cytometry A. 2008; 73(11):975-83. DOI: 10.1002/cyto.a.20643. View

Tanaka T, Halicka H, Traganos F, Seiter K, Darzynkiewicz Z . Induction of ATM activation, histone H2AX phosphorylation and apoptosis by etoposide: relation to cell cycle phase. Cell Cycle. 2007; 6(3):371-6. DOI: 10.4161/cc.6.3.3835. View

Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R . Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011; 332(6030):687-96. PMC: 3273988. DOI: 10.1126/science.1198704. View

Kuckuck F, Edwards B, Sklar L . High throughput flow cytometry. Cytometry. 2001; 44(1):83-90. View

Suni M, Dunn H, Orr P, de Laat R, Sinclair E, Ghanekar S . Performance of plate-based cytokine flow cytometry with automated data analysis. BMC Immunol. 2003; 4:9. PMC: 200973. DOI: 10.1186/1471-2172-4-9. View

10.

Ochatt S . Flow cytometry in plant breeding. Cytometry A. 2008; 73(7):581-98. DOI: 10.1002/cyto.a.20562. View

11.

ZELL T, Khoruts A, Ingulli E, Bonnevier J, Mueller D, Jenkins M . Single-cell analysis of signal transduction in CD4 T cells stimulated by antigen in vivo. Proc Natl Acad Sci U S A. 2001; 98(19):10805-10. PMC: 58555. DOI: 10.1073/pnas.191567898. View

12.

Krutzik P, Nolan G . Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry A. 2003; 55(2):61-70. DOI: 10.1002/cyto.a.10072. View

13.

Sachs K, Gentles A, Youland R, Itani S, Irish J, Nolan G . Characterization of patient specific signaling via augmentation of Bayesian networks with disease and patient state nodes. Annu Int Conf IEEE Eng Med Biol Soc. 2009; 2009:6624-7. PMC: 3124088. DOI: 10.1109/IEMBS.2009.5332563. View

14.

Wlodkowic D, Telford W, Skommer J, Darzynkiewicz Z . Apoptosis and beyond: cytometry in studies of programmed cell death. Methods Cell Biol. 2011; 103:55-98. PMC: 3263828. DOI: 10.1016/B978-0-12-385493-3.00004-8. View

15.

Zhang P, Zhao N, Zeng Z, Chang C, Zu Y . Combination of an aptamer probe to CD4 and antibodies for multicolored cell phenotyping. Am J Clin Pathol. 2010; 134(4):586-93. DOI: 10.1309/AJCP55KQYWSGZRKC. View

16.

De Rosa S, Brenchley J, Roederer M . Beyond six colors: a new era in flow cytometry. Nat Med. 2003; 9(1):112-7. DOI: 10.1038/nm0103-112. View

17.

Tarnok A, Ulrich H, Bocsi J . Phenotypes of stem cells from diverse origin. Cytometry A. 2009; 77(1):6-10. DOI: 10.1002/cyto.a.20844. View

18.

Bodenmiller B, Zunder E, Finck R, Chen T, Savig E, Bruggner R . Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators. Nat Biotechnol. 2012; 30(9):858-67. PMC: 3627543. DOI: 10.1038/nbt.2317. View

19.

Wlodkowic D, Skommer J, Darzynkiewicz Z . Cytometry in cell necrobiology revisited. Recent advances and new vistas. Cytometry A. 2010; 77(7):591-606. PMC: 2975392. DOI: 10.1002/cyto.a.20889. View

20.

Hanahan D, Weinberg R . Hallmarks of cancer: the next generation. Cell. 2011; 144(5):646-74. DOI: 10.1016/j.cell.2011.02.013. View