Magnetoluminescent Light Switches--dual Modality in DNA Detection

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2013 May 23

PMID 23692333

Citations 8

Authors

Eric D Smolensky

Katie L Peterson

Evan A Weitz

Cutler Lewandowski

Valerie C Pierre

Affiliations

Soon will be listed here.

Abstract

The synthesis and properties of two responsive magnetoluminescent iron oxide nanoparticles for dual detection of DNA by MRI and luminescence spectroscopy are presented. These magnetoluminescent agents consist of iron oxide nanoparticles conjugated with metallointercalators via a polyethylene glycol linker. Two metallointercalators were investigated: Ru(bpy')(phen)(dppz), which turns on upon DNA intercalation, and Eu-DOTA-Phen, which turns off. The characteristic light-switch responses of the metallointercalators are not affected by the iron oxide nanoparticles; upon binding to DNA the luminescence of the ruthenium complexes increases by ca. 20-fold, whereas that of the europium complex is >95% quenched. Additionally, the 17-20 nm magnetite cores, having permeable PEG coatings and stable dopamide anchors, render the two constructs efficient responsive contrast agents for MRI with unbound longitudinal and transverse relaxivities of 12.4-9.2 and 135-128 mM(-1)(Fe)s(-1), respectively. Intercalation of the metal complexes in DNA results in the formation of large clusters of nanoparticles with a resultant decrease of both r1 and r2 by 32-63% and 24-38%, respectively. The potential application of these responsive magnetoluminescent assemblies and their reversible catch-and-release properties for the purification of DNA is presented.

Citing Articles

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References

Bogdanov Jr A, Matuszewski L, Bremer C, Petrovsky A, Weissleder R . Oligomerization of paramagnetic substrates result in signal amplification and can be used for MR imaging of molecular targets. Mol Imaging. 2003; 1(1):16-23. DOI: 10.1162/15353500200200001. View

Weitz E, Chang J, Rosenfield A, Pierre V . A selective luminescent probe for the direct time-gated detection of adenosine triphosphate. J Am Chem Soc. 2012; 134(39):16099-102. DOI: 10.1021/ja304373u. View

Sun S, Zeng H, Robinson D, Raoux S, Rice P, Wang S . Monodisperse MFe2O4 (M = Fe, Co, Mn) nanoparticles. J Am Chem Soc. 2004; 126(1):273-9. DOI: 10.1021/ja0380852. View

Friedman A, Kumar C, Turro N, Barton J . Luminescence of ruthenium(II) polypyridyls: evidence for intercalative binding to Z-DNA. Nucleic Acids Res. 1991; 19(10):2595-602. PMC: 328175. View

Zhao M, Josephson L, Tang Y, Weissleder R . Magnetic sensors for protease assays. Angew Chem Int Ed Engl. 2003; 42(12):1375-8. DOI: 10.1002/anie.200390352. View

Smolensky E, Park H, Berquo T, Pierre V . Surface functionalization of magnetic iron oxide nanoparticles for MRI applications - effect of anchoring group and ligand exchange protocol. Contrast Media Mol Imaging. 2011; 6(4):189-99. PMC: 3123420. DOI: 10.1002/cmmi.417. View

Cha E, Jang E, Sun I, Lee I, Ko J, Kim Y . Development of MRI/NIRF 'activatable' multimodal imaging probe based on iron oxide nanoparticles. J Control Release. 2011; 155(2):152-8. DOI: 10.1016/j.jconrel.2011.07.019. View

Bobba G, Frias J, Parker D . Highly emissive, nine-coordinate enantiopure lanthanide complexes incorporating tetraazatriphenylenes as probes for DNA. Chem Commun (Camb). 2002; (8):890-1. DOI: 10.1039/b201451n. View

Bobba G, Bretonniere Y, Frias J, Parker D . Enantiopure lanthanide complexes incorporating a tetraazatriphenylene sensitiser and three naphthyl groups: exciton coupling, intramolecular energy transfer, efficient singlet oxygen formation and perturbation by DNA binding. Org Biomol Chem. 2003; 1(11):1870-2. DOI: 10.1039/b303085g. View

10.

Josephson L, Perez J, Weissleder R . Magnetic Nanosensors for the Detection of Oligonucleotide Sequences. Angew Chem Int Ed Engl. 2018; 40(17):3204-3206. DOI: 10.1002/1521-3773(20010903)40:17<3204::AID-ANIE3204>3.0.CO;2-H. View

11.

Xi P, Cheng K, Sun X, Zeng Z, Sun S . Magnetic Fe3O4 nanoparticles coupled with a fluorescent Eu complex for dual imaging applications. Chem Commun (Camb). 2012; 48(24):2952-4. DOI: 10.1039/c2cc18122c. View

12.

Perez J, OLoughin T, Simeone F, Weissleder R, Josephson L . DNA-based magnetic nanoparticle assembly acts as a magnetic relaxation nanoswitch allowing screening of DNA-cleaving agents. J Am Chem Soc. 2002; 124(12):2856-7. DOI: 10.1021/ja017773n. View

13.

Li M, Chen Z, Yam V, Zu Y . Multifunctional ruthenium(II) polypyridine complex-based core-shell magnetic silica nanocomposites: magnetism, luminescence, and electrochemiluminescence. ACS Nano. 2009; 2(5):905-12. DOI: 10.1021/nn800123w. View

14.

Veiseh O, Sun C, Fang C, Bhattarai N, Gunn J, Kievit F . Specific targeting of brain tumors with an optical/magnetic resonance imaging nanoprobe across the blood-brain barrier. Cancer Res. 2009; 69(15):6200-7. PMC: 2742601. DOI: 10.1158/0008-5472.CAN-09-1157. View

15.

Holmlin R, Dandliker P, Barton J . Synthesis of metallointercalator-DNA conjugates on a solid support. Bioconjug Chem. 1999; 10(6):1122-30. DOI: 10.1021/bc9900791. View

16.

Smolensky E, Marjanska M, Pierre V . A responsive particulate MRI contrast agent for copper(I): a cautionary tale. Dalton Trans. 2012; 41(26):8039-46. PMC: 3695634. DOI: 10.1039/c2dt30416c. View

17.

Kunzmann A, Andersson B, Vogt C, Feliu N, Ye F, Gabrielsson S . Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells. Toxicol Appl Pharmacol. 2011; 253(2):81-93. DOI: 10.1016/j.taap.2011.03.011. View

18.

Smolensky E, Park H, Zhou Y, Rolla G, Marjanska M, Botta M . Scaling Laws at the Nano Size: The Effect of Particle Size and Shape on the Magnetism and Relaxivity of Iron Oxide Nanoparticle Contrast Agents. J Mater Chem B. 2013; 1(22):2818-2828. PMC: 3695630. DOI: 10.1039/C3TB00369H. View

19.

Zhang L, Liu B, Dong S . Bifunctional nanostructure of magnetic core luminescent shell and its application as solid-state electrochemiluminescence sensor material. J Phys Chem B. 2007; 111(35):10448-52. DOI: 10.1021/jp0734427. View

20.

Jenkins Y, Friedman A, Turro N, Barton J . Characterization of dipyridophenazine complexes of ruthenium(II): the light switch effect as a function of nucleic acid sequence and conformation. Biochemistry. 1992; 31(44):10809-16. DOI: 10.1021/bi00159a023. View