Differential Detection of Echinococcus Spp. Copro-DNA by Nested-PCR in Domestic and Wild Definitive Hosts in Moghan Plain, Iran

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2013 May 18

PMID 23682268

Citations 6

Authors

I Mobedi

M Zare-Bidaki

Mr Siavashi

Sr Naddaf

Eb Kia

M Mahmoudi

Affiliations

Soon will be listed here.

Abstract

Background: Despite Echinococcus granulosus, there are merely two old reports of E. multilocularis infection among Iranian canids of Moghan Plain, the only area known endemic for the species. We detected specific DNA markers in fecal samples by PCR (Copro-PCR) for differential diagnosis of Echinococcus species in living canids.

Methods: Totally 144 fecal samples from domestic dogs, red foxes and a golden jackal were examined for genus-specific Echinococcus coproantigens using ELISA. Forty two positive or ambiguous samples were further examined for Echinococcus species-specific DNA markers by two different set of nested-PCR.

Results: Twenty five out of 144 (17.4%) animals were contaminated with E. granulosus including 14 (23.7%) domestic dogs, 10 (11.9%) red foxes and one (100%) golden jackal. But none of them harboured E. multilocularis species-specific Copro-DNA. The overall prevalence of E. granulosus and E. multilocularis infections in canids of the area was estimated to be 17.4% and 0.0%, respectively. There was a significant relation between the results of Copro-PCR and CA-ELISA.

Conclusion: The lack of E. multilocularis infection, compared to previous reports may be due to the differences in used diagnostic methods and/or recently limited territories of wild canids and altered their food resources in this particular area.

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References

Mobedi I, Bray R, ARFAA F, Movafag K . A study on the cestodes of carnivores in the northwest of Iran. J Helminthol. 1973; 47(3):277-81. DOI: 10.1017/s0022149x00026559. View

Bretagne S, Guillou J, Morand M, Houin R . Detection of Echinococcus multilocularis DNA in fox faeces using DNA amplification. Parasitology. 1993; 106 ( Pt 2):193-9. DOI: 10.1017/s0031182000074990. View

Knapp J, Guislain M, Bart J, Raoul F, Gottstein B, Giraudoux P . Genetic diversity of Echinococcus multilocularis on a local scale. Infect Genet Evol. 2008; 8(3):367-73. DOI: 10.1016/j.meegid.2008.02.010. View

Stefanic S, Shaikenov B, Deplazes P, Dinkel A, Torgerson P, Mathis A . Polymerase chain reaction for detection of patent infections of Echinococcus granulosus ("sheep strain") in naturally infected dogs. Parasitol Res. 2004; 92(4):347-51. DOI: 10.1007/s00436-003-1043-y. View

Zhang W, Li J, McManus D . Concepts in immunology and diagnosis of hydatid disease. Clin Microbiol Rev. 2003; 16(1):18-36. PMC: 145297. DOI: 10.1128/CMR.16.1.18-36.2003. View

Trachsel D, Deplazes P, Mathis A . Identification of taeniid eggs in the faeces from carnivores based on multiplex PCR using targets in mitochondrial DNA. Parasitology. 2007; 134(Pt 6):911-20. DOI: 10.1017/S0031182007002235. View

Monnier P, Cliquet F, Aubert M, Bretagne S . Improvement of a polymerase chain reaction assay for the detection of Echinococcus multilocularis DNA in faecal samples of foxes. Vet Parasitol. 1996; 67(3-4):185-95. DOI: 10.1016/s0304-4017(96)01039-4. View

van der Giessen J, Rombout Y, Franchimont J, Limper L, Homan W . Detection of Echinococcus multilocularis in foxes in The Netherlands. Vet Parasitol. 1999; 82(1):49-57. DOI: 10.1016/s0304-4017(98)00263-5. View

Eckert J . Predictive values and quality control of techniques for the diagnosis of Echinococcus multilocularis in definitive hosts. Acta Trop. 2003; 85(2):157-63. DOI: 10.1016/s0001-706x(02)00216-4. View

10.

Abbasi I, Branzburg A, Campos-Ponce M, Abdel Hafez S, Raoul F, Craig P . Copro-diagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence. Am J Trop Med Hyg. 2003; 69(3):324-30. View

11.

Smith G, Gangadharan B, Taylor Z, Laurenson M, Bradshaw H, Hide G . Prevalence of zoonotic important parasites in the red fox (Vulpes vulpes) in Great Britain. Vet Parasitol. 2003; 118(1-2):133-42. DOI: 10.1016/j.vetpar.2003.09.017. View

12.

Mathis A, Deplazes P, Eckert J . An improved test system for PCR-based specific detection of Echinococcus multilocularis eggs. J Helminthol. 1996; 70(3):219-22. DOI: 10.1017/s0022149x00015443. View

13.

Zhang Y, Bart J, Giraudoux P, Craig P, Vuitton D, Wen H . Morphological and molecular characteristics of Echinococcus multilocularis and Echinococcus granulosus mixed infection in a dog from Xinjiang, China. Vet Parasitol. 2006; 139(1-3):244-8. DOI: 10.1016/j.vetpar.2006.03.003. View

14.

Zare-Bidaki M, Mobedi I, Ahari S, Habibizadeh S, Naddaf S, Siavashi M . Prevalence of zoonotic intestinal helminths of canids in moghan plain, northwestern iran. Iran J Parasitol. 2012; 5(2):42-51. PMC: 3279836. View

15.

Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T . Detection of Echinococcus multilocularis in the definitive host: coprodiagnosis by PCR as an alternative to necropsy. J Clin Microbiol. 1998; 36(7):1871-6. PMC: 104943. DOI: 10.1128/JCM.36.7.1871-1876.1998. View

16.

Eslami A, Hosseini S . Echinococcus granulosus infection of farm dogs of Iran. Parasitol Res. 1998; 84(3):205-7. DOI: 10.1007/s004360050383. View

17.

Rokni M . The present status of human helminthic diseases in Iran. Ann Trop Med Parasitol. 2008; 102(4):283-95. DOI: 10.1179/136485908X300805. View

18.

Mobedi I, Sadighian A . Echinococcus multilocularis Leuckart, 1863, in red foxes, Vulpes vulpes Linn., in Moghan, Azerbaijan Province, northwest of Iran. J Parasitol. 1971; 57(3):493. View

19.

Sadjjadi S . Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int. 2005; 55 Suppl:S197-202. DOI: 10.1016/j.parint.2005.11.030. View

20.

Dyachenko V, Beck E, Pantchev N, Bauer C . Cost-effective method of DNA extraction from taeniid eggs. Parasitol Res. 2008; 102(4):811-3. DOI: 10.1007/s00436-007-0855-6. View