Polymerase Chain Reaction for Detection of Patent Infections of Echinococcus Granulosus ("sheep Strain") in Naturally Infected Dogs

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2004 Jan 17

PMID 14727186

Citations 54

Authors

Sasa Stefanic

Block S Shaikenov

Peter Deplazes

Anke Dinkel

Paul R Torgerson

Alexander Mathis

Affiliations

Soon will be listed here.

Abstract

Polymerase chain reaction (PCR) for the identification of eggs of the tapeworm Echinococcus granulosus ("sheep strain") was evaluated with primers derived from mitochondrial sequences. Specificity of these primers was confirmed by investigating DNA of other strains of E. granulosus and of 14 helminth species which inhabit the intestines of dogs. This PCR assay was used to investigate 131 purged dogs from Kazakhstan. Eighteen dogs harboured Echinococcus worms, ten of them in mixed infections with Taenia spp. Coproantigen detection was positive in 15 and taeniid eggs could be recovered from 13 of these specimens. Eight of the egg-containing samples were positive in the PCR for E. granulosus and four in a Echinococcus multilocularis -specific PCR revealing one mixed infection. Egg-containing faeces from two dogs harbouring both Taenia spp. and Echinococcus spp. were negative in both PCRs. The combination of egg isolation and PCR will also be of value in epidemiological studies when investigating environmental samples.

Citing Articles

Rapid Discriminative Identification of the Two Predominant Echinococcus Species from Canine Fecal Samples in the Tibetan Region of China by Loop-Mediated Isothermal Amplification-Lateral Flow Dipstick Assay.

Lv X, Ai J, Mo X, Ding H, Litchev S, Lu E Trop Med Infect Dis. 2024; 9(6).

PMID: 38922048 PMC: 11209407. DOI: 10.3390/tropicalmed9060136.

Dynamics of protozoal excretion in the faeces of calves during the first 28 days after arrival at the fattening farm indicate infection before regrouping and show poor temporal correlation with diarrhoea.

Bauer J, Kaske M, Oehm A, Schnyder M Parasit Vectors. 2023; 16(1):338.

PMID: 37759304 PMC: 10523781. DOI: 10.1186/s13071-023-05911-0.

Increased prevalence of canine echinococcosis a decade after the discontinuation of a governmental deworming program in Tierra del Fuego, Southern Chile.

Eisenman E, Uhart M, Kusch A, Vila A, Vanstreels R, Mazet J Zoonoses Public Health. 2022; 70(3):213-222.

PMID: 36514826 PMC: 10085837. DOI: 10.1111/zph.13017.

First detection of in Bosnia and Herzegovina.

Omeragic J, Goletic T, Softic A, Goletic S, Kapo N, Klaric Soldo D Int J Parasitol Parasites Wildl. 2022; 19:269-272.

PMID: 36406035 PMC: 9672389. DOI: 10.1016/j.ijppaw.2022.11.005.

Genetic diversity of spp. in wild carnivorous animals in Kazakhstan.

Uakhit R, Smagulova A, Syzdykova A, Abdrakhmanov S, Kiyan V Vet World. 2022; 15(6):1489-1496.

PMID: 35993082 PMC: 9375211. DOI: 10.14202/vetworld.2022.1489-1496.

References

Stieger C, Hegglin D, SCHWARZENBACH G, Mathis A, Deplazes P . Spatial and temporal aspects of urban transmission of Echinococcus multilocularis. Parasitology. 2002; 124(Pt 6):631-40. DOI: 10.1017/s0031182002001749. View

Cabrera M, Canova S, Rosenzvit M, Guarnera E . Identification of Echinococcus granulosus eggs. Diagn Microbiol Infect Dis. 2002; 44(1):29-34. DOI: 10.1016/s0732-8893(02)00414-5. View

Torgerson P, Karaeva R, Corkeri N, Abdyjaparov T, Kuttubaev O, Shaikenov B . Human cystic echinococcosis in Kyrgystan: an epidemiological study. Acta Trop. 2002; 85(1):51-61. DOI: 10.1016/s0001-706x(02)00257-7. View

Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T . Detection of Echinococcus multilocularis in the definitive host: coprodiagnosis by PCR as an alternative to necropsy. J Clin Microbiol. 1998; 36(7):1871-6. PMC: 104943. DOI: 10.1128/JCM.36.7.1871-1876.1998. View

Bowles J, Blair D, McManus D . A molecular phylogeny of the genus Echinococcus. Parasitology. 1995; 110 ( Pt 3):317-28. DOI: 10.1017/s0031182000080902. View

Torgerson P, Shaikenov B, Baitursinov K, Abdybekova A . The emerging epidemic of echinococcosis in Kazakhstan. Trans R Soc Trop Med Hyg. 2002; 96(2):124-8. DOI: 10.1016/s0035-9203(02)90276-2. View

ONeill S, Giordano R, Colbert A, Karr T, Robertson H . 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci U S A. 1992; 89(7):2699-702. PMC: 48729. DOI: 10.1073/pnas.89.7.2699. View

Mathis A, Deplazes P, Eckert J . An improved test system for PCR-based specific detection of Echinococcus multilocularis eggs. J Helminthol. 1996; 70(3):219-22. DOI: 10.1017/s0022149x00015443. View

Malgor R, Nonaka N, Basmadjian I, Sakai H, Carambula B, Oku Y . Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay. Int J Parasitol. 1998; 27(12):1605-12. DOI: 10.1016/s0020-7519(97)00127-6. View

10.

Celi F, Zenilman M, Shuldiner A . A rapid and versatile method to synthesize internal standards for competitive PCR. Nucleic Acids Res. 1993; 21(4):1047. PMC: 309252. DOI: 10.1093/nar/21.4.1047. View

11.

Deplazes P, Gottstein B, Eckert J, Jenkins D, Ewald D, Jimenez-Palacios S . Detection of Echinococcus coproantigens by enzyme-linked immunosorbent assay in dogs, dingoes and foxes. Parasitol Res. 1992; 78(4):303-8. DOI: 10.1007/BF00937088. View

12.

Craig P, Macpherson C, Nelson G . The identification of eggs of Echinococcus by immunofluorescence using a specific anti-oncospheral monoclonal antibody. Am J Trop Med Hyg. 1986; 35(1):152-8. DOI: 10.4269/ajtmh.1986.35.152. View

13.

Deplazes P, Dinkel A, Mathis A . Molecular tools for studies on the transmission biology of Echinococcus multilocularis. Parasitology. 2004; 127 Suppl:S53-61. DOI: 10.1017/s0031182003003500. View

14.

Christofi G, Deplazes P, Christofi N, Tanner I, Economides P, Eckert J . Screening of dogs for Echinococcus granulosus coproantigen in a low endemic situation in Cyprus. Vet Parasitol. 2002; 104(4):299-306. DOI: 10.1016/s0304-4017(01)00637-9. View

15.

Thompson R, McManus D . Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002; 18(10):452-7. DOI: 10.1016/s1471-4922(02)02358-9. View

16.

Torgerson P, Budke C . Echinococcosis--an international public health challenge. Res Vet Sci. 2003; 74(3):191-202. DOI: 10.1016/s0034-5288(03)00006-7. View

17.

Abbasi I, Branzburg A, Campos-Ponce M, Abdel Hafez S, Raoul F, Craig P . Copro-diagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence. Am J Trop Med Hyg. 2003; 69(3):324-30. View