Structural Modulation of the Biological Activity of Gold Nanoparticles Functionalized with a Carbonic Anhydrase Inhibitor

Overview

Journal Eur Phys J E Soft Matter

Publisher EDP Sciences

Specialty Biophysics

Date 2013 May 17

PMID 23677447

Citations 2

Authors

Francesca Bellissima

Fabrizio Carta

Alessio Innocenti

Andrea Scozzafava

Piero Baglioni

Claudiu T Supuran

Debora Berti

Affiliations

Soon will be listed here.

Abstract

Gold nanoparticles (AuNPs) have gained attention for their potential and application in different fields, e.g. nanomedicine. This study explores the surface functionalization of AuNP with inhibitors of carbonic anhydrases (CAs, EC 4.2.1.1). Some CA transmembrane isoforms have been recognized as therapeutic targets for the treatment of hypoxic tumors. Embedding a CA inhibitory function onto a nanosized unit has been proved to enable selective targeting of transmembrane isoforms. We report the preparation in aqueous media, the characterization and CA inhibition tests of AuNPs coated with a sulfonamide (SA) derivative, already known for its inhibitory activity toward CAs. The physico-chemical characterization of SA-coated AuNPs was performed with a combination of scattering and spectroscopic techniques. We detect a threshold effect of the SA concentration on the final hydrodynamic and core sizes of the capped nanoparticles and on their stability over aggregation. These modified nanoparticles were assayed for inhibition of some CA transmembrane isoforms (CA IX and XII) as well as of two cytosolic isoforms (CA I and II), and show interesting inhibitory efficiency in the submicromolar range and some selectivity for transmembrane isoforms.

Citing Articles

The reversed intra- and extracellular pH in tumors as a unified strategy to chemotherapeutic delivery using targeted nanocarriers.

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PMID: 34522586 PMC: 8424227. DOI: 10.1016/j.apsb.2021.01.012.

Multivalent Carbonic Anhydrases Inhibitors.

Carta F, Dumy P, Supuran C, Winum J Int J Mol Sci. 2019; 20(21).

PMID: 31661796 PMC: 6862271. DOI: 10.3390/ijms20215352.

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