MiR-200 and MiR-96 Families Repress Neural Induction from Human Embryonic Stem Cells

Overview

Journal Development

Specialty Biology

Date 2013 May 3

PMID 23637338

Citations 37

Authors

Zhong-Wei Du

Li-Xiang Ma

Christian Phillips

Su-Chun Zhang

Affiliations

Soon will be listed here.

Abstract

The role of miRNAs in neuroectoderm specification is largely unknown. We screened miRNA profiles that are differentially changed when human embryonic stem cells (hESCs) were differentiated to neuroectodermal precursors (NEP), but not to epidermal (EPI) cells and found that two miRNA families, miR-200 and miR-96, were uniquely downregulated in the NEP cells. We confirmed zinc-finger E-box-binding homeobox (ZEB) transcription factors as a target of the miR-200 family members and identified paired box 6 (PAX6) transcription factor as the new target of miR-96 family members via gain- and loss-of-function analyses. Given the essential roles of ZEBs and PAX6 in neural induction, we propose a model by which miR-200 and miR-96 families coordinate to regulate neural induction.

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