Biological Activity and Magnetic Resonance Imaging of Superparamagnetic Iron Oxide Nanoparticles-labeled Adipose-derived Stem Cells

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2013 Apr 27

PMID 23618360

Citations 14

Authors

Jingjing Fan

Yanbin Tan

Liyong Jie

Xinying Wu

Risheng Yu

Minming Zhang

Affiliations

Soon will be listed here.

Abstract

Introduction: No comparative study of adipose-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) by using superparamagnetic iron oxide nanoparticles (SPIOs)-labeling and magnetic resonance imaging (MRI) has been performed.

Methods: We studied the biological activity and MRI of ADSCs by labeling them with SPIOs and comparing them with BMSCs. After incubating the cells in culture medium with different levels of SPIOs (control group: 0 μg/ml; Groups 1 to 3: 25, 50, and 100 μg/ml) for 24 hours, we compared ADSCs with BMSCs in terms of intracellular iron content, labeling efficiency, and cell viability. Stem cells in the culture medium containing 50 μg/ml SPIOs were induced into osteoblasts and fat cells. Adipogenic and osteogenic differentiation potentials were compared. R2* values of MRI in vitro were compared.

Results: The results showed that labeling efficiency was highest in Group 2. Intracellular iron content and R2* values increased with increasing concentrations of SPIOs, whereas cell viability decreased with increasing concentrations of SPIOs, and adipogenic and osteogenic differentiation potentials decreased. However, we found no significant difference between the two kinds of cells for any of these indexes.

Conclusions: ADSCs can be labeled and traced as easily as BMSCs in vitro. Given their abundance and higher proliferative capacity, as was previously shown, ADSCs may be better suited to stem cell therapy than are BMSCs.

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References

Balakumaran A, Pawelczyk E, Ren J, Sworder B, Chaudhry A, Sabatino M . Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their "stemness". PLoS One. 2010; 5(7):e11462. PMC: 2898800. DOI: 10.1371/journal.pone.0011462. View

Wimpenny I, Markides H, El Haj A . Orthopaedic applications of nanoparticle-based stem cell therapies. Stem Cell Res Ther. 2012; 3(2):13. PMC: 3392773. DOI: 10.1186/scrt104. View

Rice H, Hsu E, Sheng H, Evenson D, Freemerman A, Safford K . Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice. AJR Am J Roentgenol. 2007; 188(4):1101-8. DOI: 10.2214/AJR.06.0663. View

Chen Y, Hsiao J, Liu H, Lai I, Yao M, Hsu S . The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells. Toxicol Appl Pharmacol. 2010; 245(2):272-9. DOI: 10.1016/j.taap.2010.03.011. View

Wang L, Deng J, Wang J, Xiang B, Yang T, Gruwel M . Superparamagnetic iron oxide does not affect the viability and function of adipose-derived stem cells, and superparamagnetic iron oxide-enhanced magnetic resonance imaging identifies viable cells. Magn Reson Imaging. 2008; 27(1):108-19. DOI: 10.1016/j.mri.2008.05.013. View

Odintsov B, Chun J, Mulligan J, Berry S . 14.1 T whole body MRI for detection of mesoangioblast stem cells in a murine model of Duchenne muscular dystrophy. Magn Reson Med. 2011; 66(6):1704-14. DOI: 10.1002/mrm.22942. View

ZuK P, Zhu M, Ashjian P, De Ugarte D, Huang J, Mizuno H . Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13(12):4279-95. PMC: 138633. DOI: 10.1091/mbc.e02-02-0105. View

Kostura L, Kraitchman D, Mackay A, Pittenger M, Bulte J . Feridex labeling of mesenchymal stem cells inhibits chondrogenesis but not adipogenesis or osteogenesis. NMR Biomed. 2004; 17(7):513-7. DOI: 10.1002/nbm.925. View

Backesjo C, Li Y, Lindgren U, Haldosen L . Activation of Sirt1 decreases adipocyte formation during osteoblast differentiation of mesenchymal stem cells. J Bone Miner Res. 2006; 21(7):993-1002. DOI: 10.1359/jbmr.060415. View

10.

Kedziorek D, Kraitchman D . Superparamagnetic iron oxide labeling of stem cells for MRI tracking and delivery in cardiovascular disease. Methods Mol Biol. 2010; 660:171-83. PMC: 3096997. DOI: 10.1007/978-1-60761-705-1_11. View

11.

Pawelczyk E, Arbab A, Pandit S, Hu E, Frank J . Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging. NMR Biomed. 2006; 19(5):581-92. DOI: 10.1002/nbm.1038. View

12.

Kern S, Eichler H, Stoeve J, Kluter H, Bieback K . Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-301. DOI: 10.1634/stemcells.2005-0342. View

13.

Kim H, Oh S, Joo H, Son K, Song I, Moon W . The effects of clinically used MRI contrast agents on the biological properties of human mesenchymal stem cells. NMR Biomed. 2010; 23(5):514-22. DOI: 10.1002/nbm.1487. View

14.

Racuciu M, Creanga D, Airinei A . Citric-acid-coated magnetite nanoparticles for biological applications. Eur Phys J E Soft Matter. 2006; 21(2):117-21. DOI: 10.1140/epje/i2006-10051-y. View

15.

Frolich K, Scherzed A, Mlynski R, Technau A, Hagen R, Kleinsasser N . Multipotent stromal cells for autologous cell therapy approaches in the guinea pig model. ORL J Otorhinolaryngol Relat Spec. 2010; 73(1):9-16. DOI: 10.1159/000320598. View

16.

Mitchell J, McIntosh K, Zvonic S, Garrett S, Floyd Z, Kloster A . Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells. 2005; 24(2):376-85. DOI: 10.1634/stemcells.2005-0234. View

17.

Suh J, Lee J, Choi Y, Yu F, Yang V, Lee S . Efficient labeling of mesenchymal stem cells using cell permeable magnetic nanoparticles. Biochem Biophys Res Commun. 2008; 379(3):669-75. DOI: 10.1016/j.bbrc.2008.12.041. View

18.

Apopa P, Qian Y, Shao R, Guo N, Schwegler-Berry D, Pacurari M . Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling. Part Fibre Toxicol. 2009; 6:1. PMC: 2632982. DOI: 10.1186/1743-8977-6-1. View

19.

Heyn C, Ronald J, Ramadan S, Snir J, Barry A, Mackenzie L . In vivo MRI of cancer cell fate at the single-cell level in a mouse model of breast cancer metastasis to the brain. Magn Reson Med. 2006; 56(5):1001-10. DOI: 10.1002/mrm.21029. View

20.

Dash R, Chung J, Chan T, Yamada M, Barral J, Nishimura D . A molecular MRI probe to detect treatment of cardiac apoptosis in vivo. Magn Reson Med. 2011; 66(4):1152-62. PMC: 3138815. DOI: 10.1002/mrm.22876. View