Lumenal Peroxisomal Protein Aggregates Are Removed by Concerted Fission and Autophagy Events

Overview

Journal Autophagy

Specialty Cell Biology

Date 2013 Apr 26

PMID 23614977

Citations 22

Authors

Selvambigai Manivannan

Rinse de Boer

Marten Veenhuis

Ida J van der Klei

Affiliations

Soon will be listed here.

Abstract

We demonstrated that in the yeast Hansenula polymorpha peroxisome fission and degradation are coupled processes that are important to remove intra-organellar protein aggregates. Protein aggregates were formed in peroxisomes upon synthesis of a mutant catalase variant. We showed that the introduction of these aggregates in the peroxisomal lumen had physiological disadvantages as it affected growth and caused enhanced levels of reactive oxygen species. Formation of the protein aggregates was followed by asymmetric peroxisome fission to separate the aggregate from the mother organelle. Subsequently, these small, protein aggregate-containing organelles were degraded by autophagy. In line with this observation we showed that the degradation of the protein aggregates was strongly reduced in dnm1 and pex11 cells in which peroxisome fission is reduced. Moreover, this process was dependent on Atg1 and Atg11.

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