The Impact of Proteomics on the Understanding of Functions and Biogenesis of Fungal Extracellular Vesicles

Overview

Journal J Proteomics

Publisher Elsevier

Specialty Biochemistry

Date 2013 Apr 16

PMID 23583696

Citations 56

Authors

Marcio L Rodrigues

Ernesto S Nakayasu

Igor C Almeida

Leonardo Nimrichter

Affiliations

Soon will be listed here.

Abstract

Several microbial molecules are released to the extracellular space in vesicle-like structures. In pathogenic fungi, these molecules include pigments, polysaccharides, lipids, and proteins, which traverse the cell wall in vesicles that accumulate in the extracellular space. The diverse composition of fungal extracellular vesicles (EV) is indicative of multiple mechanisms of cellular biogenesis, a hypothesis that was supported by EV proteomic studies in a set of Saccharomyces cerevisiae strains with defects in both conventional and unconventional secretory pathways. In the human pathogens Cryptococcus neoformans, Histoplasma capsulatum, and Paracoccidioides brasiliensis, extracellular vesicle proteomics revealed the presence of proteins with both immunological and pathogenic activities. In fact, fungal EV have been demonstrated to interfere with the activity of immune effector cells and to increase fungal pathogenesis. In this review, we discuss the impact of proteomics on the understanding of functions and biogenesis of fungal EV, as well as the potential role of these structures in fungal pathogenesis. This article is part of a Special Issue entitled: Trends in Microbial Proteomics.

Citing Articles

From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different Extracellular Vesicles.

Martinez-Lopez R, Molero G, Marcela Parra-Giraldo C, Cabeza M, Castejon G, Garcia-Duran C Int J Mol Sci. 2025; 26(1.

PMID: 39796100 PMC: 11720215. DOI: 10.3390/ijms26010244.

Thermotolerance in S. cerevisiae as a model to study extracellular vesicle biology.

Logan C, Staton C, Oliver J, Bouffard J, Kazmirchuk T, Magi M J Extracell Vesicles. 2024; 13(5):e12431.

PMID: 38711329 PMC: 11074623. DOI: 10.1002/jev2.12431.

Extracellular Vesicles from Mycelial Cells: Implication for Fungal-Host Interplays.

Aor A, Sangenito L, Mello T, Joffe L, Rizzo J, Veiga V J Fungi (Basel). 2024; 10(4).

PMID: 38667948 PMC: 11051067. DOI: 10.3390/jof10040277.

Corrected and republished from: "Extracellular Vesicle Formation in Impacts Fungal Virulence".

Castelli R, Pereira A, Honorato L, Valdez A, de Oliveira H, Bazioli J Infect Immun. 2024; 92(4):e0003724.

PMID: 38470135 PMC: 11003230. DOI: 10.1128/iai.00037-24.

Traversing the Cell Wall: The Chitinolytic Activity of Extracellular Vesicles Facilitates Their Release.

Valdez A, de Souza T, Artunduaga Bonilla J, Zamith-Miranda D, Piffer A, Araujo G J Fungi (Basel). 2023; 9(11).

PMID: 37998859 PMC: 10672645. DOI: 10.3390/jof9111052.

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