Preliminary Pharmacological Evaluation of Martynia Annua Linn Leaves for Wound Healing

Overview

Journal Asian Pac J Trop Biomed

Specialty Tropical Medicine

Date 2013 Apr 10

PMID 23569806

Citations 7

Authors

Lodhi Santram

A K Singhai

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the wound healing potential of fractions from ethanol extract of Martynia annua (M. annua) Linn leaves.

Methods: Ethanol extract of M. annua Linn leaves was fractionate into three different fractions (MAF-A, MAF-B and MAF-C) which were screened for wound healing potential using two models: excision and incision on rats. The thin layer chromatography (TLC) profile of all fractions were analyzed and TLC of luteolin was also done. The Povidone-Iodine Ointment was used as reference for comparision. Excision and incision wounds were created on dorsal portion of rats for study. Wound contraction, biochemical parameters (protein level and hydroxyproline level) and histopathological study were performed in excision wound model whereas incision model was used for determination of tensile strength.

Results: The wound contraction and tensile strength of skin tissues were observed significantly greater in MAF-C fraction treated group than other two fractions (P<0.01). In excision wound method (on day 18) protein content and hydroxyproline were found significantly higher in MAF-C group than control group (P<0.01). Histopathological study also showed better angiogenesis, matured collagen fibres and fibroblast cells as compared with the control group.

Conclusions: In conclusion, our findings suggest that fraction MAF-C from ethanol extract of M. annua leaves is found most effective in wound healing.

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References

Diegelmann R, Evans M . Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 2004; 9:283-9. DOI: 10.2741/1184. View

Murrell G, Francis M, Bromley L . Modulation of fibroblast proliferation by oxygen free radicals. Biochem J. 1990; 265(3):659-65. PMC: 1133685. DOI: 10.1042/bj2650659. View

Sadaf F, Saleem R, Ahmed M, Ahmad S, Navaid-ul-Zafar . Healing potential of cream containing extract of Sphaeranthus indicus on dermal wounds in Guinea pigs. J Ethnopharmacol. 2006; 107(2):161-3. DOI: 10.1016/j.jep.2006.02.022. View

Shukla A, Rasik A, Patnaik G . Depletion of reduced glutathione, ascorbic acid, vitamin E and antioxidant defence enzymes in a healing cutaneous wound. Free Radic Res. 1997; 26(2):93-101. DOI: 10.3109/10715769709097788. View

Mali P, Ansari A, Chaturvedi M . Antifertility effect of chronically administered Martynia annua root extract on male rats. J Ethnopharmacol. 2002; 82(2-3):61-7. DOI: 10.1016/s0378-8741(02)00084-3. View

Cotelle N, Bernier J, Catteau J, Pommery J, Wallet J, Gaydou E . Antioxidant properties of hydroxy-flavones. Free Radic Biol Med. 1996; 20(1):35-43. DOI: 10.1016/0891-5849(95)02014-4. View

Nijveldt R, van Nood E, van Hoorn D, Boelens P, van Norren K, Van Leeuwen P . Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74(4):418-25. DOI: 10.1093/ajcn/74.4.418. View

Woessner Jr J . The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys. 1961; 93:440-7. DOI: 10.1016/0003-9861(61)90291-0. View

Kuwano H, Yano K, Ohno S, Ikebe M, Kitamura K, Toh Y . Dipyridamole inhibits early wound healing in rat skin incisions. J Surg Res. 1994; 56(3):267-70. DOI: 10.1006/jsre.1994.1042. View

10.

Silambujanaki P, Bala Tejo Chandra C, Kumar K, Chitra V . Wound healing activity of Glycosmis arborea leaf extract in rats. J Ethnopharmacol. 2010; 134(1):198-201. DOI: 10.1016/j.jep.2010.11.046. View

11.

Svobodova A, Psotova J, Walterova D . Natural phenolics in the prevention of UV-induced skin damage. A review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2004; 147(2):137-45. View

12.

Rashed A, Afifi F, Disi A . Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J Ethnopharmacol. 2003; 88(2-3):131-6. DOI: 10.1016/s0378-8741(03)00194-6. View

13.

Govindarajan R, Vijayakumar M, Rao C, Shirwaikar A, Mehrotra S, Pushpangadan P . Healing potential of Anogeissus latifolia for dermal wounds in rats. Acta Pharm. 2005; 54(4):331-8. View

14.

Inan A, Sen M, Koca C, Akpinar A, Dener C . The effect of purified micronized flavonoid fraction on the healing of anastomoses in the colon in rats. Surg Today. 2006; 36(9):818-22. DOI: 10.1007/s00595-006-3251-4. View

15.

Biswas T, Maity L, Mukherjee B . Wound healing potential of Pterocarpus santalinus linn: a pharmacological evaluation. Int J Low Extrem Wounds. 2005; 3(3):143-50. DOI: 10.1177/1534734604268385. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

Tournaire C, Croux S, Maurette M, Beck I, Hocquaux M, Braun A . Antioxidant activity of flavonoids: efficiency of singlet oxygen (1 delta g) quenching. J Photochem Photobiol B. 1993; 19(3):205-15. DOI: 10.1016/1011-1344(93)87086-3. View