Increased Expression of Factor XIII-A in Patients with Chronic Rhinosinusitis with Nasal Polyps

Overview

Journal J Allergy Clin Immunol

Specialty Allergy & Immunology

Date 2013 Apr 2

PMID 23541322

Citations 66

Authors

Tetsuji Takabayashi

Atsushi Kato

Anju T Peters

Kathryn E Hulse

Lydia A Suh

Roderick Carter

James Norton

Leslie C Grammer

Bruce K Tan

Rakesh K Chandra

David B Conley

Robert C Kern

Shigeharu Fujieda

Robert P Schleimer

Affiliations

Soon will be listed here.

Abstract

Background: Profound edema or formation of a pseudocyst containing plasma proteins is a prominent characteristic of nasal polyps (NP). However, the mechanisms underlying NP retention of plasma proteins in the submucosa remain unclear. Recently, we reported that impairment of fibrinolysis causes excessive fibrin deposition in NP and this might be involved in the retention of plasma proteins. Although the coagulation cascade plays a critical role in fibrin clot formation at extravascular sites, the expression and role of coagulation factors in NP remain unclear.

Objective: The objective of this study was to investigate the expression of coagulation factors in patients with chronic rhinosinusitis (CRS).

Methods: Sinonasal tissues were collected from patients with CRS and control subjects. We assayed mRNA for factor XIII-A (FXIII-A) by using real-time PCR and measured FXIII-A protein by means of ELISA, immunohistochemistry, and immunofluorescence.

Results: FXIII-A mRNA levels were significantly increased in NP tissue from patients with CRS with NP (P < .001) compared with uncinate tissue from patients with CRS or control subjects. Similarly, FXIII-A protein levels were increased in NP. Immunofluorescence analysis revealed that FXIII-A expression in inflammatory cells and FXIII-A(+) cell numbers were significantly increased in NP. Most FXIII-A staining was observed within CD68(+)/CD163(+) M2 macrophages in NP. Levels of FXIII-A correlated with markers of M2 macrophages, suggesting that M2 macrophages are major FXIIIA-producing cells in NP.

Conclusion: Overproduction of FXIII-A by M2 macrophages might contribute to the excessive fibrin deposition in the submucosa of NP, which might contribute to the tissue remodeling and pathogenesis of CRS with NP.

Citing Articles

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Moon S, Rha M Allergy Asthma Immunol Res. 2024; 16(6):585-600.

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High M2/M1 Macrophage Ratio Observed in Nasal Polyps Formed in Allergic Fungal Rhinosinusitis.

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[Research progress of type 2 inflammation-related tissue remodeling in nasal polyps].

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Eosinophilic Chronic Rhinosinusitis and Pathogenic Role of Protease.

Kim J, Kwak S, Lee J, Park I, Lee S, Shin J Int J Mol Sci. 2023; 24(24).

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Endotypic heterogeneity and pathogenesis in chronic rhinosinusitis.

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