Linkage Mapping and Molecular Diversity at the Flower Sex Locus in Wild and Cultivated Grapevine Reveal a Prominent SSR Haplotype in Hermaphrodite Plants

Overview

Journal Mol Biotechnol

Publisher Springer

Specialties Biotechnology
Molecular Biology

Date 2013 Mar 28

PMID 23532385

Citations 18

Authors

Juri Battilana

Silvia Lorenzi

Flavia M Moreira

Paula Moreno-Sanz

Osvaldo Failla

Francesco Emanuelli

M Stella Grando

Affiliations

Soon will be listed here.

Abstract

Cultivars used for wine and table grape have self-fertile hermaphrodite flowers whereas wild European vines and American and Asian species are dioecious, having either male or female flowers. Consistent with previous studies, the flower sex trait was mapped as a single major locus on chromosome 2 based on a pure Vitis vinifera population segregating for hermaphrodite and female progeny, and a hybrid population producing all three flower sex types. The sex locus was placed between the same SSR and SNP markers on both genetic maps, although abnormal segregation hampered to fine map the genomic region. From a total of 55 possible haplotypes inferred for three SSR markers around the sex locus, in a population of 132 V. sylvestris accessions and 171 V. vinifera cultivars, one of them accounted for 66 % of the hermaphrodite individuals and may be the result of domestication. Specific size variants of the VVIB23 microsatellite sequence within the 3'-UTR of a putative YABBY1 gene were found to be statistically significantly associated with the sex alleles M, H and f; these markers can provide assistance in defining the status of wild grapevine germplasm.

Citing Articles

Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map.

Alahakoon D, Fennell A, Helget Z, Bates T, Karn A, Manns D Plants (Basel). 2022; 11(5).

PMID: 35270166 PMC: 8912348. DOI: 10.3390/plants11050696.

Gene is Associated With Sex Determination in .

Men Y, Li J, Shen H, Yang Y, Fan S, Li K Front Genet. 2022; 12:727260.

PMID: 35003203 PMC: 8733387. DOI: 10.3389/fgene.2021.727260.

VviPLATZ1 is a major factor that controls female flower morphology determination in grapevine.

Iocco-Corena P, Chaib J, Torregrosa L, Mackenzie D, Thomas M, Smith H Nat Commun. 2021; 12(1):6995.

PMID: 34848714 PMC: 8632994. DOI: 10.1038/s41467-021-27259-8.

Multiple independent recombinations led to hermaphroditism in grapevine.

Zou C, Massonnet M, Minio A, Patel S, Llaca V, Karn A Proc Natl Acad Sci U S A. 2021; 118(15).

PMID: 33837155 PMC: 8053984. DOI: 10.1073/pnas.2023548118.

The wild grape genome sequence provides insights into the transition from dioecy to hermaphroditism during grape domestication.

Badouin H, Velt A, Gindraud F, Flutre T, Dumas V, Vautrin S Genome Biol. 2020; 21(1):223.

PMID: 32892750 PMC: 7487632. DOI: 10.1186/s13059-020-02131-y.

References

Lowe K, Walker M . Genetic linkage map of the interspecific grape rootstock cross Ramsey (Vitis champinii) x Riparia Gloire (Vitis riparia). Theor Appl Genet. 2006; 112(8):1582-92. DOI: 10.1007/s00122-006-0264-8. View

Carmona M, Chaib J, Martinez-Zapater J, Thomas M . A molecular genetic perspective of reproductive development in grapevine. J Exp Bot. 2008; 59(10):2579-96. DOI: 10.1093/jxb/ern160. View

Chaib J, Torregrosa L, Mackenzie D, Corena P, Bouquet A, Thomas M . The grape microvine - a model system for rapid forward and reverse genetics of grapevines. Plant J. 2010; 62(6):1083-92. DOI: 10.1111/j.1365-313X.2010.04219.x. View

De Andres M, Benito A, Perez-Rivera G, Ocete R, Lopez M, Gaforio L . Genetic diversity of wild grapevine populations in Spain and their genetic relationships with cultivated grapevines. Mol Ecol. 2011; 21(4):800-16. DOI: 10.1111/j.1365-294X.2011.05395.x. View

Barnaud A, Lacombe T, Doligez A . Linkage disequilibrium in cultivated grapevine, Vitis vinifera L. Theor Appl Genet. 2006; 112(4):708-16. DOI: 10.1007/s00122-005-0174-1. View

Boualem A, Fergany M, Fernandez R, Troadec C, Martin A, Morin H . A conserved mutation in an ethylene biosynthesis enzyme leads to andromonoecy in melons. Science. 2008; 321(5890):836-8. DOI: 10.1126/science.1159023. View

Sefc K, Regner F, Turetschek E, Glossl J, Steinkellner H . Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome. 1999; 42(3):367-73. View

Myles S, Boyko A, Owens C, Brown P, Grassi F, Aradhya M . Genetic structure and domestication history of the grape. Proc Natl Acad Sci U S A. 2011; 108(9):3530-5. PMC: 3048109. DOI: 10.1073/pnas.1009363108. View

This P, Lacombe T, Thomas M . Historical origins and genetic diversity of wine grapes. Trends Genet. 2006; 22(9):511-9. DOI: 10.1016/j.tig.2006.07.008. View

10.

Marguerit E, Boury C, Manicki A, Donnart M, Butterlin G, Nemorin A . Genetic dissection of sex determinism, inflorescence morphology and downy mildew resistance in grapevine. Theor Appl Genet. 2009; 118(7):1261-78. DOI: 10.1007/s00122-009-0979-4. View

11.

Bandelt H, Forster P, Rohl A . Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol. 1999; 16(1):37-48. DOI: 10.1093/oxfordjournals.molbev.a026036. View

12.

Prunet N, Morel P, Negrutiu I, Trehin C . Time to stop: flower meristem termination. Plant Physiol. 2009; 150(4):1764-72. PMC: 2719151. DOI: 10.1104/pp.109.141812. View

13.

Dalbo M, Ye G, Weeden N, Steinkellner H, Sefc K, Reisch B . A gene controlling sex in grapevines placed on a molecular marker-based genetic map. Genome. 2000; 43(2):333-40. View

14.

Fechter I, Hausmann L, Daum M, Rosleff Sorensen T, Viehover P, Weisshaar B . Candidate genes within a 143 kb region of the flower sex locus in Vitis. Mol Genet Genomics. 2012; 287(3):247-59. DOI: 10.1007/s00438-012-0674-z. View

15.

Di Gaspero G, Cipriani G, Adam-Blondon A, Testolin R . Linkage maps of grapevine displaying the chromosomal locations of 420 microsatellite markers and 82 markers for R-gene candidates. Theor Appl Genet. 2007; 114(7):1249-63. DOI: 10.1007/s00122-007-0516-2. View

16.

Costantini L, Battilana J, Lamaj F, Fanizza G, Grando M . Berry and phenology-related traits in grapevine (Vitis vinifera L.): from quantitative trait loci to underlying genes. BMC Plant Biol. 2008; 8:38. PMC: 2395262. DOI: 10.1186/1471-2229-8-38. View

17.

Riaz S, Krivanek A, Xu K, Walker M . Refined mapping of the Pierce's disease resistance locus, PdR1, and Sex on an extended genetic map of Vitis rupestris x V. arizonica. Theor Appl Genet. 2006; 113(7):1317-29. DOI: 10.1007/s00122-006-0385-0. View

18.

Zhang J, Hausmann L, Eibach R, Welter L, Topfer R, Zyprian E . A framework map from grapevine V3125 (Vitis vinifera 'Schiava grossa' x 'Riesling') x rootstock cultivar 'Börner' (Vitis riparia x Vitis cinerea) to localize genetic determinants of phylloxera root resistance. Theor Appl Genet. 2009; 119(6):1039-51. DOI: 10.1007/s00122-009-1107-1. View

19.

Stephens M, Donnelly P . A comparison of bayesian methods for haplotype reconstruction from population genotype data. Am J Hum Genet. 2003; 73(5):1162-9. PMC: 1180495. DOI: 10.1086/379378. View