Temporal Appearance of Opsonizing Antibody to Francisella Tularensis: Detection by a Radiometabolic Assay

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1975 Mar 1

PMID 234915

Citations 5

Authors

P G Canonico

A T McManus

J A Mangiafico

L S Sammons

V G MCGANN

H G DANGERFIELD

Affiliations

Soon will be listed here.

Abstract

The burst in oxidative metabolism that is mediated through activation of the hexose monophosphate shunt and accompanies particle ingestion by polymorphonuclear leukocytes was used as the indicator in an in vitro radiometabolic assay for detection of specific opsonizing antibody to Francisella tularensis. Release of 14CO2 from radiolabeled glucose was increased significantly when specific immune serum added to suspensions of monkey polymorphonuclear leukocytes and F. tularensis. With this method, opsonizing antibodies to F. tularensis were detected in monkey serum 3 days after vaccination. Significantly increased opsonic activity in these monkeys preceded the appearance of, and persisted longer than, antibody activity as determined by conventional serological techniques. In addition, sera from 11 of 12 humans that were immunized 1 month to 13 years previously and had nondiagnostic agglutinating antibody titers demonstrated significant opsonizing activity.

Citing Articles

Demonstration of opsonizing antibodies to Francisella tularensis by leukocyte chemiluminescence.

Lofgren S, Tarnvik A, Carlsson J Infect Immun. 1980; 29(2):329-34.

PMID: 7216416 PMC: 551121. DOI: 10.1128/iai.29.2.329-334.1980.

Phagocytosis and killing of Francisella tularensis by human polymorphonuclear leukocytes.

Lofgren S, Tarnvik A, BLOOM G, Sjoberg W Infect Immun. 1983; 39(2):715-20.

PMID: 6832815 PMC: 348008. DOI: 10.1128/iai.39.2.715-720.1983.

A wild and an attenuated strain of Francisella tularensis differ in susceptibility to hypochlorous acid: a possible explanation of their different handling by polymorphonuclear leukocytes.

Lofgren S, Tarnvik A, Thore M, Carlsson J Infect Immun. 1984; 43(2):730-4.

PMID: 6319290 PMC: 264361. DOI: 10.1128/iai.43.2.730-734.1984.

Susceptibility of mice treated with cyclophosphamide to lethal infection with Leptospira interrogans Serovar pomona.

Adler B, Faine S Infect Immun. 1976; 14(3):703-8.

PMID: 965093 PMC: 420943. DOI: 10.1128/iai.14.3.703-708.1976.

Antibody response to Rocky Mountain spotted fever.

Kenyon R, Canonico P, Sammons L, Bagley L, Pedersen Jr C J Clin Microbiol. 1976; 3(5):513-8.

PMID: 819455 PMC: 274340. DOI: 10.1128/jcm.3.5.513-518.1976.

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