An Improved Method for the Extraction and Quantification of Adult Echinococcus from Wildlife Definitive Hosts

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2013 Mar 9

PMID 23471781

Citations 12

Authors

Karen Gesy

Michael Pawlik

Luciene Kapronczai

Brent Wagner

Brett Elkin

Helen Schwantje

Emily Jenkins

Affiliations

Soon will be listed here.

Abstract

The scraping and counting technique (SCT), with sensitivity values close to 100 %, has been the protocol recommended by global regulatory bodies for the extraction of Echinococcus cestodes from the intestines of wild carnivores. The proposed scraping, filtration and counting technique (SFCT) maintained the sensitivity (p = 0.801, α = 0.05) and increased the efficiency of sample processing. SCT had sensitivity and negative predictive value of 91 and 97 %, respectively, when compared to SFCT. The SFCT significantly decreased processing time (p = 0.0001, α = 0.05) for each sample. The SFCT took an average of 68.5 min less to quantify than SCT, as the SFCT samples consistently contained less debris. The SFCT is therefore appealing for general post-mortem surveillance, to determine if prevalence and intensity of infection are changing in an established region, or if these important parasitic zoonoses are newly established in a region or host species.

Citing Articles

Age-dependent relationships among diet, body condition, and Echinococcus multilocularis infection in urban coyotes.

Sugden S, Steckler D, Sanderson D, Abercrombie B, Abercrombie D, Seguin M PLoS One. 2023; 18(8):e0290755.

PMID: 37647321 PMC: 10468061. DOI: 10.1371/journal.pone.0290755.

NOVEL REPORT OF THE EUROPEAN VARIANT OF ECHINOCOCCUS MULTILOCULARIS IN COYOTES (CANIS LATRANS) IN NEW YORK STATE.

Conlon C, Schuler K, Lejeune M, Whipps C J Parasitol. 2023; 109(4):357-361.

PMID: 37527278 PMC: 10658865. DOI: 10.1645/22-104.

It's a small world for parasites: evidence supporting the North American invasion of European .

Santa M, Umhang G, Klein C, Grant D, Ruckstuhl K, Musiani M Proc Biol Sci. 2023; 290(1994):20230128.

PMID: 36883278 PMC: 9993045. DOI: 10.1098/rspb.2023.0128.

A highly endemic area of Echinococcus multilocularis identified through a comparative re-assessment of prevalence in the red fox (Vulpes vulpes), Alto Adige (Italy: 2019-2020).

Obber F, Celva R, Da Rold G, Trevisiol K, Ravagnan S, Danesi P PLoS One. 2022; 17(5):e0268045.

PMID: 35511816 PMC: 9070940. DOI: 10.1371/journal.pone.0268045.

Host and geographic differences in prevalence and diversity of gastrointestinal helminths of foxes (), coyotes () and wolves () in Québec, Canada.

Bouchard E, Schurer J, Kolapo T, Wagner B, Masse A, Locke S Int J Parasitol Parasites Wildl. 2021; 16:126-137.

PMID: 34552844 PMC: 8441108. DOI: 10.1016/j.ijppaw.2021.09.002.

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