Intraovarian Transplantation of Primordial Follicles Fails to Rescue Chemotherapy Injured Ovaries

Overview

Journal Sci Rep

Specialty Science

Date 2013 Mar 7

PMID 23463338

Citations 19

Authors

Mi-Ryung Park

Yun-Jung Choi

Deug-Nam Kwon

Chankyu Park

Hong-Thuy Bui

Sangiliyandi Gurunathan

Ssang-Goo Cho

Hyuk Song

Han Geuk Seo

Gyesik Min

Jin-Hoi Kim

Affiliations

Soon will be listed here.

Abstract

Busulfan and cyclophosphamide (B/C)-treated mice exhibited a marked increase in apoptosis and a concomitant decrease in the ovarian weight. Histological and RT-PCR analysis indicate that the period of germ cell depletion in the B/C-treated ovaries coincides with decreased expression of genes Figla, Lhx8, Nobox, c-kit, and Sox3. However, depletion of the ovarian germ cells is mediated by autophagy-independent pathways that involve Fas/FasL-, TNF-, and/or p53-signalings. Treatment with B/C resulted in the cease of the reproductive function to produce their offspring during the 15(th) week post-treatment period in female mice. Furthermore, injection of the 3 × 10(6) GFP positive primordial follicles into the ovaries of the B/C treated mouse did not show apparent colonization of the transplanted follicles within the recipient ovaries. The present results suggest that B/C treatment is closely associated with an increased risk of premature ovarian failure.

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