Prognostic Implications of MicoRNA MiR-195 Expression in Human Tongue Squamous Cell Carcinoma

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Mar 2

PMID 23451060

Citations 28

Authors

Ling-Fei Jia

Su-Bi Wei

Kai Gong

Ye-Hua Gan

Guang-Yan Yu

Affiliations

Soon will be listed here.

Abstract

Background: miR-195 is aberrantly expressed in multiple types of disease. But little is known about the dysregulation of miR-195 in tongue squamous cell carcinoma (TSCC). In this study, we investigated the roles of miR-195 in the development and progression of TSCC.

Methods: Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we evaluated miR-195 expression in TSCC samples from 81 patients. Overall survival of these patients was examined using Kaplan-Meier curves with log-rank tests and the Cox proportional hazards model. The expression of two known miR-195 target genes, Cyclin D1 and Bcl-2, was also examined in the TSCC samples by immunohistochemistry. The effects of miR-195 overexpression on cell cycle progression and apoptosis and its effects on the expression of Cyclin D1 and Bcl-2 were examined in transfected TSCC cell lines (SCC-15 and Cal27) using fluorescence-activated cell sorting assays, luciferase reporter assays, and Western blots.

Results: Reduced miR-195 expression was associated with tumor size and the clinical stage of TSCC tumors. Kaplan-Meier survival analysis indicated that the TSCC patients with reduced expression of miR-195 had poor overall survival and in multivariable analyses low levels of miR-195 emerged as an independent prognostic factor for this clinical outcome. Levels of miR-195 expression were inversely correlated with the expression of Cyclin D1 and Bcl-2. Overexpression of miR-195 inhibited cell cycle progression, promoted apoptosis, and reduced Cyclin D1 and Bcl-2 expression in two TSCC cell lines.

Conclusions: miR-195 may have potential applications as a prognostic factor for TSCC patients.

Citing Articles

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PMID: 39913046 DOI: 10.1007/s12094-025-03854-9.

MicroRNA expression as a prognostic biomarker of tongue squamous cell carcinoma (TSCC): a systematic review and meta-analysis.

Sun Y, Li Y, Zhou W, Liu Z BMC Oral Health. 2024; 24(1):406.

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A Novel LncRNA MASCC1 Regulates the Progression and Metastasis of Head and Neck Squamous Cell Carcinoma by Sponging miR-195.

Wang Y, Qin Z, Chen Y, Zheng Y, Jia L Cancers (Basel). 2023; 15(24).

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Is the Non-Coding RNA miR-195 a Biodynamic Marker in the Pathogenesis of Head and Neck Squamous Cell Carcinoma? A Prognostic Meta-Analysis.

Dioguardi M, Spirito F, Caloro G, Lo Muzio L, Cantore S, Ballini A J Pers Med. 2023; 13(2).

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