Mapping and Analysis of Phosphorylation Sites: a Quick Guide for Cell Biologists

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2013 Mar 1

PMID 23447708

Citations 134

Authors

Noah Dephoure

Kathleen L Gould

Steven P Gygi

Douglas R Kellogg

Affiliations

Soon will be listed here.

Abstract

A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.

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