Zymography Methods for Visualizing Hydrolytic Enzymes

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2013 Feb 28

PMID 23443633

Citations 113

Authors

Jennifer Vandooren

Nathalie Geurts

Erik Martens

Philippe E Van den Steen

Ghislain Opdenakker

Affiliations

Soon will be listed here.

Abstract

Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful, but often misinterpreted, tool yielding information on potential hydrolytic activities, enzyme forms and the locations of active enzymes. In this Review, zymography techniques are compared in terms of advantages, limitations and interpretations. With in gel zymography, enzyme forms are visualized according to their molecular weights. Proteolytic activities are localized in tissue sections with in situ zymography. In vivo zymography can pinpoint proteolytic activity to sites in an intact organism. Future development of novel substrate probes and improvement in detection and imaging methods will increase the applicability of zymography for (reverse) degradomics studies.

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