A DNA-binding Protein is Required for Termination of Transcription by RNA Polymerase I in Xenopus Laevis

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1990 Jun 1

PMID 2342463

Citations 25

Authors

B McStay

R H Reeder

Affiliations

Soon will be listed here.

Abstract

We describe a partially fractionated in vitro transcription system from Xenopus laevis for the assay of transcription termination by RNA polymerase I. Termination in vitro was found to require a specific terminator sequence in the DNA and a DNA-binding protein fraction that produces a footprint over the terminator sequence.

Citing Articles

Functional architecture of the Reb1-Ter complex of Schizosaccharomyces pombe.

Jaiswal R, Choudhury M, Zaman S, Singh S, Santosh V, Bastia D Proc Natl Acad Sci U S A. 2016; 113(16):E2267-76.

PMID: 27035982 PMC: 4843429. DOI: 10.1073/pnas.1525465113.

Survey and summary: transcription by RNA polymerases I and III.

Paule M, White R Nucleic Acids Res. 2000; 28(6):1283-98.

PMID: 10684922 PMC: 111039. DOI: 10.1093/nar/28.6.1283.

Histone acetyltransferase and protein kinase activities copurify with a putative Xenopus RNA polymerase I holoenzyme self-sufficient for promoter-dependent transcription.

Albert A, Denton M, Kermekchiev M, Pikaard C Mol Cell Biol. 1998; 19(1):796-806.

PMID: 9858602 PMC: 83936. DOI: 10.1128/MCB.19.1.796.

In vivo analyses of RNA polymerase I termination in Schizosaccharomyces pombe.

Melekhovets Y, Shwed P, Nazar R Nucleic Acids Res. 1998; 25(24):5103-9.

PMID: 9396822 PMC: 147157. DOI: 10.1093/nar/25.24.5103.

The Xenopus RNA polymerase I transcription factor, UBF, has a role in transcriptional enhancement distinct from that at the promoter.

McStay B, Sullivan G, Cairns C EMBO J. 1997; 16(2):396-405.

PMID: 9029158 PMC: 1169644. DOI: 10.1093/emboj/16.2.396.

References

Galas D, Schmitz A . DNAse footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res. 1978; 5(9):3157-70. PMC: 342238. DOI: 10.1093/nar/5.9.3157. View

LABHART P, Reeder R . A point mutation uncouples RNA 3'-end formation and termination during ribosomal gene transcription in Xenopus laevis. Genes Dev. 1990; 4(2):269-76. DOI: 10.1101/gad.4.2.269. View

Grummt I, Maier U, Ohrlein A, Hassouna N, Bachellerie J . Transcription of mouse rDNA terminates downstream of the 3' end of 28S RNA and involves interaction of factors with repeated sequences in the 3' spacer. Cell. 1985; 43(3 Pt 2):801-10. DOI: 10.1016/0092-8674(85)90253-3. View

LABHART P, Reeder R . Characterization of three sites of RNA 3' end formation in the Xenopus ribosomal gene spacer. Cell. 1986; 45(3):431-43. DOI: 10.1016/0092-8674(86)90329-6. View

Grummt I, Rosenbauer H, Niedermeyer I, Maier U, Ohrlein A . A repeated 18 bp sequence motif in the mouse rDNA spacer mediates binding of a nuclear factor and transcription termination. Cell. 1986; 45(6):837-46. DOI: 10.1016/0092-8674(86)90558-1. View

Deuschle U, Gentz R, Bujard H . lac Repressor blocks transcribing RNA polymerase and terminates transcription. Proc Natl Acad Sci U S A. 1986; 83(12):4134-7. PMC: 323685. DOI: 10.1073/pnas.83.12.4134. View

Kadonaga J, Tjian R . Affinity purification of sequence-specific DNA binding proteins. Proc Natl Acad Sci U S A. 1986; 83(16):5889-93. PMC: 386402. DOI: 10.1073/pnas.83.16.5889. View

Platt T . Transcription termination and the regulation of gene expression. Annu Rev Biochem. 1986; 55:339-72. DOI: 10.1146/annurev.bi.55.070186.002011. View

Henderson S . A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA gene. Cell. 1986; 47(6):891-900. DOI: 10.1016/0092-8674(86)90804-4. View

10.

Grummt I, Kuhn A, Bartsch I, Rosenbauer H . A transcription terminator located upstream of the mouse rDNA initiation site affects rRNA synthesis. Cell. 1986; 47(6):901-11. DOI: 10.1016/0092-8674(86)90805-6. View

11.

McStay B, Reeder R . A termination site for Xenopus RNA polymerase I also acts as an element of an adjacent promoter. Cell. 1986; 47(6):913-20. DOI: 10.1016/0092-8674(86)90806-8. View

12.

LABHART P, Reeder R . Ribosomal precursor 3' end formation requires a conserved element upstream of the promoter. Cell. 1987; 50(1):51-7. DOI: 10.1016/0092-8674(87)90661-1. View

13.

LABHART P, Reeder R . A 12-base-pair sequence is an essential element of the ribosomal gene terminator in Xenopus laevis. Mol Cell Biol. 1987; 7(5):1900-5. PMC: 365294. DOI: 10.1128/mcb.7.5.1900-1905.1987. View

14.

Christianson T, Clayton D . A tridecamer DNA sequence supports human mitochondrial RNA 3'-end formation in vitro. Mol Cell Biol. 1988; 8(10):4502-9. PMC: 365525. DOI: 10.1128/mcb.8.10.4502-4509.1988. View

15.

Walker P, Reeder R . The Xenopus laevis ribosomal gene promoter contains a binding site for nuclear factor-1. Nucleic Acids Res. 1988; 16(22):10657-68. PMC: 338931. DOI: 10.1093/nar/16.22.10657. View

16.

Bartsch I, Schoneberg C, Grummt I . Purification and characterization of TTFI, a factor that mediates termination of mouse ribosomal DNA transcription. Mol Cell Biol. 1988; 8(9):3891-7. PMC: 365448. DOI: 10.1128/mcb.8.9.3891-3897.1988. View

17.

Connelly S, Manley J . A CCAAT box sequence in the adenovirus major late promoter functions as part of an RNA polymerase II termination signal. Cell. 1989; 57(4):561-71. DOI: 10.1016/0092-8674(89)90126-8. View

18.

Kruse B, Narasimhan N, Attardi G . Termination of transcription in human mitochondria: identification and purification of a DNA binding protein factor that promotes termination. Cell. 1989; 58(2):391-7. DOI: 10.1016/0092-8674(89)90853-2. View

19.

Pikaard C, McStay B, SCHULTZ M, Bell S, Reeder R . The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF. Genes Dev. 1989; 3(11):1779-88. DOI: 10.1101/gad.3.11.1779. View

20.

Moss T . A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis. Nature. 1983; 302(5905):223-8. DOI: 10.1038/302223a0. View